| 鸡核受体辅激活蛋白基因(NCOA1)SNPs分析及mRNA的表达 |
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| 引用本文： | 黎寿丰,赵振华,黄华云,李春苗,张静,薛龙岗.鸡核受体辅激活蛋白基因(NCOA1)SNPs分析及mRNA的表达[J].农业生物技术学报,2011,19(5). |
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| 作者姓名： | 黎寿丰 赵振华 黄华云 李春苗 张静 薛龙岗 |
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| 作者单位： | 中国农业科学院家禽研究所,扬州,225003 |
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| 基金项目： | 江苏省“333”工程培养资金项目(No.BRA2010162); 现代农业产业技术体系建设专项资金; 中国农业科学院家禽所博士基金(No.JQ2009002)共同资助 |
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| 摘 要： | 核受体辅激活蛋白基因(NCOA1)是类固醇激素受体超家族中的一员,在生殖细胞的生长和分化过程中起着重要的调节作用。本研究利用PCR-SSCP技术对NCOA1基因外显子的多态性进行了分析,在第3和12外显子上分别发现了SNPs位点,并采用实时荧光定量PCR对多态性位点处mRNA表达水平进行了研究,发现NCOA1基因随着生长发育其表达量呈波动性增加,14周龄前表达量很少,随着性成熟NCOA1基因表达量快速增加,28周龄时其表达量达到峰值,之后表达量下降。产蛋性能较好的母系鸡NCOA1基因的相对表达量比产蛋性能较差的父系鸡较早地开始增加,并且在14周龄后表达量显著高于父系鸡(P<0.05)。同位点对产蛋性能有显著影响的优势基因型AA和CC个体的表达量在16周后也显著高于同位点的其他基因型个体(P<0.05)。研究提示NCOA1基因mRNA表达水平影响鸡的性成熟过程和产蛋性能。
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| 关 键 词： | 鸡 核受体辅激活蛋白基因(NCOA1) PCR-SSCP 实时荧光定量PCR |
SNPs Analysis and mRNA Expression Level of Nuclear receptor Co- activator 1 Gene (NCOA1) in Chicken |
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| Li Shoufeng,Zhao Zhenhua,Huang Huayun,Li Chunmiao,Zhang Jing,Xue Longgang.SNPs Analysis and mRNA Expression Level of Nuclear receptor Co- activator 1 Gene (NCOA1) in Chicken[J].Journal of Agricultural Biotechnology,2011,19(5). |
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| Authors: | Li Shoufeng Zhao Zhenhua Huang Huayun Li Chunmiao Zhang Jing Xue Longgang |
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| Institution: | Li Shoufeng* Zhao Zhenhua Huang Huayun Li Chunmiao Zhang Jing Xue Longgang Poultry Institute,Chinese Academy of Agricultural Sciences,Yangzhou 225003,China |
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| Abstract: | Nuclear receptor co-activator 1 gene (NCOA1) is one of the members of steroid hormone receptor which is essential for growth and differentiation of ovarian follicles. Single nucleotide polymorphisms (SNPs) were detected by PCR-SSCP, and two SNPs was found at exon 3 and 12. Rea1-time quantitative PCR was used for identification of expression changes of NCOA1 gene at exon 3 and 12. Results indicated that NCOA1 gene expression was increased along with growth as undulatory property and few before 14 weeks. NCOA... |
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| Keywords: | Chicken Nuclear receptor co-activator 1 gene(NCOA1) PCR-SSCP Real-time quantitative PCR |
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