几种生长调节物质及有机附加物对菠萝愈伤组织分化影响的细胞学研究

用菠萝6－74冠芽诱导愈伤组织，在愈伤组织分化过程中进行细胞学研究，观察到菠萝伤组织中薄壁细胞随机分化形成胚性细胞，胚性细胞分裂形成两种分生组织即生细胞团和分生组织结节，它们可以进一步形成器官原基，分化为芽、根、苄氨基嘌呤（BA）对务组织芽的分化起决定性作用，它刺激分生细胞团产生单向极性生长形成芽原基。萘乙酸（NAA）则对根的分化起决定作用，玉米素（ZT）在有BA和NAA的存在下加速了细胞型分裂和分生组织向芽原基的分化，水解乳蛋白（LH）促进芽的分化，但却抑制维管组织结节向根的转化；。酵母提取液（YE）则对两种分生组织的进一步分化都有抑制作用。

A CYTOLOGICAL STUDY OF THE EFFECT OF GROWTH REGULATORS ANDADDITIVE ORGANIC SUBSTANCES ON CALLUS DIFFERENTIATION OFTHE PINEAPPLE (Ananas comosus Merr. )

Cytological observations showed that random dedifferentiation of parenchymatous cells produced embryogenic cells and then gave rise to two types of meristems. They were meristematic cell aggregations and meristematic nodules, which might further form organs primordia. BA had a determinative effect on bud differentiation of the callus, initiating growth showing meristiatic monopolarity leading to bud primordium formation. NAA had a important effect on root differentiation. With NAA and BA, ZT accelerated cell division and organ primodium rormation. LH accelerated bud differentiation but inhibited root formation from vascular nodules. And YE restrained further differentiation of both meristems.