Detection of tumorigenic Agrobacterium strains from infected apple saplings by colony PCR with improved PCR primers |
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| Authors: | Kouichi Suzaki Kouji Yoshida Hiroyuki Sawada |
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| Institution: | (1) Department of Apple Research, National Institute of Fruit Tree Science, Shimokuriyagawa, Morioka 020-0123, Japan;(2) Department of Plant Protection, National Institute of Fruit Tree Science, Tsukuba, Japan;(3) National Institute for Agro-Environmental Sciences, Tsukuba, Japan |
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| Abstract: | With the colony polymerase chain reaction (PCR), the specificity of newly prepared primer sets VCF2/VCR2, VCF3/VCR3, VCF4/VCR4, and VCF5/VCR5 to Ti or Ri plasmids in Agrobacterium strains were compared to that of the conventional set VCF/VCR. At first, control strains, which consisted of a nonpathogenic strain and phytopathogenic strains carrying Ti or Ri plasmid, were used. VCF3/VCR3 and VCF5/VCR5 were highly specific to all the phytopathogenic strains, whereas the others were not. These two primer sets were superior to VCF/VCR in their specificity with colony PCR to tumorigenic Agrobacterium strains isolated from apple saplings. |
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| Keywords: | Agrobacterium spp Colony PCR Ti or Ri plasmid Crown gall Apple |
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