| 4种基因型烟草叶肉原生质体培养的研究 |
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| 引用本文： | 陈名红,李天飞,陈学军,吴渝生,李大春.4种基因型烟草叶肉原生质体培养的研究[J].江西农业大学学报,2006,28(2):175-179. |
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| 作者姓名： | 陈名红 李天飞 陈学军 吴渝生 李大春 |
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| 作者单位： | 云南民族大学,化学与生物技术学院,云南,昆明,650031;云南省烟草科学研究院,云南,昆明,650106;云南农业大学,农学与生物技术学院,云南,昆明,650201 |
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| 摘 要： | 以K326、云85、红大、G-28等普通烟草品种的叶片为起始材料进行原生质体培养的研究。结果表明,基因型对烟草叶肉原生质体的培养有明显影响作用,并对影响烟草叶肉原生质体的分离、培养和再生植株等环节的其它相关因素进行了研究和探索。
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| 关 键 词： | 烟草 原生质体培养 植株再生 影响因素 |
| 文章编号： | 1000-2286（2006）02-0175-05 |
| 修稿时间： | 2005年9月16日 |
A Study on Mesophyll Protoplast Culture of Four Genotypes of Tabacco |
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| CHEN Ming-hong,LI Tian-fei,CHEN Xue-jun,WU Yu-sheng,LI Da-chun.A Study on Mesophyll Protoplast Culture of Four Genotypes of Tabacco[J].Acta Agriculturae Universitis Jiangxiensis,2006,28(2):175-179. |
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| Authors: | CHEN Ming-hong LI Tian-fei CHEN Xue-jun WU Yu-sheng LI Da-chun |
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| Institution: | 1. College of Chemistry and Bio - Science of YNU, Kunming 650031, China; 2. Yunnan Academy of Tabacco Science, Kunming 650106, China; 3. College of Agronomy and Biotechnology of YAU, Kunming 650201, China |
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| Abstract: | Tobacco protoplast culture was studied with mesophyll protoplasts isolated from K326,Yun85,Hongda and G-28.The obtained results showed that genotype affected tobacco protoplast culture obviously.Some other factors affecting protoplast isolation,protoplast culture and plant regeneration were also studied. |
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| Keywords: | tabacco protoplast culture plant regeneration influencing factor |
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