应用酯酶同工酶研究加拿大一枝黄花和栽培品种黄莺的亲缘关系(英文)

采用聚丙烯酰胺凝胶电泳法(PAGE)对来自6处不同生境的加拿大一枝黄花(Solidago canadensisL.)和栽培品种黄莺(Solidago goldenwings)进行了酯酶同工酶测试、分析;并通过对酶谱特征进行数量化分级,根据欧氏距离及类平均法(UP-GMA)对6个供试样品进行聚类分析.结果表明:(1)6个样品都具有2条迁移率分别为0.62和0.66的酶带;(2)供试样品间共出现8种不同带型的酶谱表型差异显著,其中不同生境加拿大一枝黄花均聚成一组,显示其酶谱表型与生态型也一致,而栽培品种黄莺则单独成为1组.(3)加拿大一枝黄花与园艺栽培品种黄莺的亲缘关系较远.因此,采用酯酶同工酶测试方法,可以鉴别来自不同生境的加拿大一枝黄花及栽培品种黄莺.

A study on genetic relationship between Solidago canadensis L. and cultivar species S.goldenwings by esterase isozymes

Esterase isozymes in Solidago canadensis L.and S.goldenwings from six habitats were studied using vertical slab polyacrylamide gel electrophoresis.The zymogram variations were significant among those plants.According to the quantitative data of the characteristics of enzyme spectrum,the six plants were systematically clustered using UPGMA method.The results were as follows:(1) all tested plants had two same bands(Rf 0.62 and Rf 0.66);(2)eight types of band were presented in the tested plants,all tested plants of S.canadensis were clustered into one group,while the other group only contained one species,S.goldenwings,the plants of S.canadensis collected from the same place were clustered together,showing that their zymogram phenotypes showed almost the same as their ecotype in the region;(3) the phylogenetic relationships of S.canadensis were far away from S.goldenwings.So esterase isozymes could be used as an effective biochemical marker in S.canadensis sampled from various habitats to distinguish S.canadensis from S.goldenwings.