| 检测鸡传染性支气管炎病毒抗体的三种血清学方法的比较 |
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| 引用本文： | 徐辉,Ｊ．Ｅ．Ｌｏｈｒ. 检测鸡传染性支气管炎病毒抗体的三种血清学方法的比较[J]. 中国预防兽医学报, 1998, 0(3) |
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| 作者姓名： | 徐辉 Ｊ．Ｅ．Ｌｏｈｒ |
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| 作者单位： | 浙江省农业科学院病毒实验室(徐辉),德国奥伦多夫国家兽医调查中心(Ｊ．Ｅ．Ｌｏｈｒ) |
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| 摘 要： | 应用酶联免疫吸附试验（ＥＬＩＳＡ）、琼脂扩散沉淀试验（ＡＧＰ）和鸡气管环培养中和试验（ＳＮｉｎＴＯＣｓ）三种常规血清学方法对实验鸡血样的鸡传染性支气管炎病毒抗体进行了检测。从实验鸡血样的检测结果表明,ＥＬＩＳＡ和气管环中和试验的灵敏度较好,而ＡＧＰ的灵敏度相对较差,但三者均有较好的特异性。对田间送检血样的检测结果表明,ＥＬＩＳＡ与气管环中和试验、ＥＬＩＳＡ与ＡＧＰ的一致性均较好,而气管环中和试验与ＡＧＰ的一致性较差。ＥＬＩＳＡ效价与气管环中和试验效价的相关系数为０．８４
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| 关 键 词： | 鸡传染性支气管炎病毒抗体 琼扩试验 气管环培养中和试验 |
Comparison of Three Serological Tests for the Detecction of IBV antibody |
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| Abstract: | Enzyme linked immmunosorbent assay(ELISA),agar gel precipitation test(AGP)and serum neutralization in chick trachea rings cultures(SN in TOCs)were used for detection of infectious bronchitis virus(IBV)antibody in the chicken sera respectively.Results showed that ELISA and SN in TOCs had higher and AGP had lower sensitivity,all the three tests had good specificity.For the field sera,the agreements between ELISA and SN in TOCs,or between ELISA and AGP were much higher than between SN in TOCs and AGP.The correlation coefficient of ELISA titers and SN in TOCs titers was 0 84. |
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| Keywords: | IBV antibody ELISA AGP SN in TOCs |
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