| 结球甘蓝相对于大白菜的连锁群特异SSR标记建立 |
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| 引用本文： | 顾爱侠,王彦华,轩淑欣,陈雪平,申书兴. 结球甘蓝相对于大白菜的连锁群特异SSR标记建立[J]. 园艺学报, 2009, 36(8): 1221-1226 |
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| 作者姓名： | 顾爱侠 王彦华 轩淑欣 陈雪平 申书兴 |
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| 作者单位： | (河北农业大学园艺学院, 河北保定071000) |
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| 基金项目： | 国家自然科学基金，河北省自然科学基金，河北农业大学将帅计划项目 |
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| 摘 要： | 选用位于甘蓝9个连锁群的91对SSR引物, 对8个结球甘蓝品种和7个大白菜品种进行PCR扩增, 筛选出25对结球甘蓝相对大白菜的特异SSR引物, 涉及甘蓝的9个连锁群, 其中位于O1连锁群3对, O2连锁群4对, O3连锁群2对, O4连锁群3对, O5连锁群2对, O6连锁群2对, O7连锁群4对,O8连锁群2对, O9连锁群3对。为利用SSR标记鉴定大白菜—结球甘蓝异附加系中附加的结球甘蓝染色体奠定了基础。
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| 关 键 词： | 大白菜 结球甘蓝 SSR 连锁群特异标记 |
| 收稿时间： | 2009-03-24 |
| 修稿时间： | 2009-06-23 |
Establishment of Specific SSR from Different Linkage Groups of Cabbage Compared with Chinese Cabbage |
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| GU Ai-xia,WANG Yan-hua,XUAN Shu-xin,CHEN Xue-ping,SHEN Shu-xing. Establishment of Specific SSR from Different Linkage Groups of Cabbage Compared with Chinese Cabbage[J]. Acta Horticulturae Sinica, 2009, 36(8): 1221-1226 |
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| Authors: | GU Ai-xia WANG Yan-hua XUAN Shu-xin CHEN Xue-ping SHEN Shu-xing |
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| Affiliation: | (College of Horticulture, Agricultural University of Hebei,Baoding,Hebei0 71001, China) |
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| Abstract: | Ninety-one primers from 9 cabbage linkage group swere amp lified on 8 cabbage cultivars and 7 Chinese cabbage cultivars, 25 of them were specific on cabbage compared with Chinese cabbage. These primers assigned 9 linkage groups of cabbage, 3 of them were assigned linkage group O1, 4 of them were assigned linkage group O2, 2 of them were assigned linkage group O3, 3 of them were assigned linkage group O4, 2 of them were assigned linkage group O5, 2 of them were assigned linkage group O6, 4 of them were assigned linkage group O7, 2 of them were assigned linkage group O8, and 3 of them were assigned linkage group O9. It laid a foundation to further dentify the introduced chromosome of cabbage in Chinese cabbage alien addition lines. |
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| Keywords: | SSR |
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