| 猪圆环病毒Ⅱ型ORF2重组蛋白抗体诊断试剂IgG的研制 |
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| 引用本文： | 陆英杰,林涛,欧阳峰,谭铁兵,马春全,邓桦.猪圆环病毒Ⅱ型ORF2重组蛋白抗体诊断试剂IgG的研制[J].西北农业学报,2009,18(5):64-66. |
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| 作者姓名： | 陆英杰 林涛 欧阳峰 谭铁兵 马春全 邓桦 |
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| 作者单位： | 1. 佛山科学技术学院,生命科学学院,广东佛山,528231
2. 佛山市农林技术推广中心,畜牧兽医站,广东佛山,528231 |
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| 基金项目： | 广东省佛山市科技发展专项基金项目 |
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| 摘 要： | 应用猪圆环病毒Ⅱ型(Porcine circovirus 2,PCV2)ORF2重组蛋白,研制诊断PCV2的抗体试剂.利用原核表达系统表达PCV2 ORF2蛋白,经12%浓度的SDS-PAGE凝胶电泳分析后免疫家兔制备抗PCV2 ORF2重组蛋白抗体,并用间接酶联免疫吸附试验(indirect-ELISA)确定其效价,然后以Western blotting法分析该抗体特异性.结果表明:PCV2 ORF2重组蛋白被表达,且在免疫家兔后获得效价高、特异性好的抗体.兔抗PCV2-ORF2重组蛋白抗体以其特异性好的特点可以作为免疫诊断试剂.
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| 关 键 词： | 猪圆环病毒Ⅱ型 抗体诊断试剂IgG |
| 收稿时间： | 2008/12/22 0:00:00 |
| 修稿时间： | 2009/3/20 0:00:00 |
Trituration of Antibodies Diagnostic Reagent IgG against PCV2-ORF2 Recombinant Protein |
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| LU Yingjie,LIN Tao,OUYANG Feng,TAN Tiebing,MA Chunquan and DENG Hua.Trituration of Antibodies Diagnostic Reagent IgG against PCV2-ORF2 Recombinant Protein[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(5):64-66. |
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| Authors: | LU Yingjie LIN Tao OUYANG Feng TAN Tiebing MA Chunquan and DENG Hua |
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| Institution: | The Veterinary Department of Life Science Institute, Foshan Scientific Technology College, Foshan Guangdong 528231, China;The Veterinary Department of Life Science Institute, Foshan Scientific Technology College, Foshan Guangdong 528231, China;The Veterinary Station of Farming and Forest Technology Spreading Center, Foshan City, Foshan Guangdong 528231, China;The Veterinary Station of Farming and Forest Technology Spreading Center, Foshan City, Foshan Guangdong 528231, China;The Veterinary Department of Life Science Institute, Foshan Scientific Technology College, Foshan Guangdong 528231, China;The Veterinary Department of Life Science Institute, Foshan Scientific Technology College, Foshan Guangdong 528231, China |
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| Abstract: | PCV2-ORF2 recombinant protein was used and prepared immunodiagnosis antibody agent for PCV2.The structural protein encoded by PCV2-ORF2 was expressed in recombinant E.col BL21 (DE3) and inclusion body analyzed by 12% SDS-PAGE then immunized rabbits to prepare PCV2-ORF2 reco mbinant protein antibody and its titer was tested by indirect-ELISA then the antibody specificity analyzed by Western blotting.The result showed PCV2-ORF2 recom binant protein product expressed then obtained highest titer and fine specificity antibody by immunized rabbits.Antibody of rabbits against PCV2-ORF2 recom binant protein could be made immunodiagnosis reagent for its fine specificity. |
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| Keywords: | ORF2 |
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