一株氮磷营养素降解菌的筛选和鉴定

[目的]筛选能够高效降解氮和磷营养素的菌株。[方法]以尿素为唯一氮源,以STTP为唯一的磷源,配制氮磷选择性培养基,对生活污水和一些工业废水中的微生物进行分离培养和筛选,采用显微镜观察技术和分子生物学技术相结合对筛选出的菌株进行了鉴定。[结果]通过分离和筛选,从生活污水和工业废水中得到了一株能够利用STTP和尿素作为能源的菌株lyh6A。该菌株在较高浓度尿素(4.00 mg/ml)和STTP(0.50 mg/ml)选择性培养基上生长良好。根据菌落的形态、菌株显微形态及18S rDNA序列同源比较结果,确定所筛选出的菌株lyh6A与曲霉属杂色曲霉等亲缘关系较近,18S rDNA序列相似性达到99%以上。[结论]该研究将为利用微生物进行氮、磷富营养化污染水体的治理提供基础。

Screening and Identification of a Nitrogen-phosphorus Degradation Strain

[Objective] The study aimed to screen the strain that could highly degrade nitrogen and phosphorus nutrients.[Method] With urea as the only nitrogen origin and STTP as the only phosphorus origin,they were prepared as the N-P selective medium to isolate,culture and screen the microbe from the domestic sewage and industrial wastewater and the screened strain was identified by combining the microscope inspection technique and molecular biotechnology.[Result] Through isolation and screening,a strain lyh6A that could use the urea and STTP as the energy origin was obtained from the domestic sewage and industrial wastewater.This strain could grow well on the selective medium with high concn.of urea at 4.00 mg/ml and STTP at 0.50 mg/ml.Based on the morphology of the colony and micro-morphology of the strain and the homologous comparison of 18S rDNA sequence,it was confirmed that the screened strain lyh6A had closer genetic relationship with Aspergillus versicolor etc,with over 99% similarity in 18S rDNA sequence.[Conclusion] This study could give support to the treatment of water contaminated by nitrogen and phosphorus nutrients.