氟苯尼考在感染鲁氏耶尔森氏菌的西伯利亚鲟体内的药效学

通过构建鲁氏耶尔森氏菌(Yersinia ruckeri)感染西伯利亚鲟(Acipenser baeri)病理模型,设立阴性对照组(不攻菌不给药)及阳性对照组(攻菌不给药),比较研究了高(50 mg.kg-1)、中(20 mg.kg-1)、低(5 mg.kg-1)氟苯尼考剂量治疗组和氯霉素(10 mg.kg-1)对照组西伯利亚鲟体内的药效学。连续给药3天后,阴性对照组和阳性对照组累计死亡率分别为0%和50%,表明病理模型构建成功;而氟苯尼考低、中、高剂量组和治疗对照组累计死亡率分别为30%、0%、0%、10%。愈后各实验组鱼血液中均无细菌感染;除阴性对照组外,鱼肝、肾组织中均可分离到鲁氏菌,但不致鱼死亡。实验结果表明,氟苯尼考对感染鲁氏耶尔森氏菌的西伯利亚鲟有较好的治疗作用,药效优于氯霉素,可用于治疗鱼类细菌性疾病。

In vivo Pharmacodynamics of Florfenicol against Pathogenic Bacterium Yersinia ruckeri in Siberian Sturgeon Acipenser baerii Challenged by the Pathogen

In vivo pharmacodynamics of high(50 mg·kg-1) / middle(20 mg·kg-1) / low(5 mg·kg-1) concentration of florfenicol and chloramphenicol(10 mg·kg-1,control group) were studied through negative control group(no bacterial infection or drug given) and positive control group(infected by bacterial but no drug given and by establishment of a pathological model in Siberia sturgeon(Acipenser baeri)infected by pathogenic bacterium Yersinia ruckeri.The cumulative mortality rates were found of 0% in negative control group and of 50% in positive control group three days after continuous oral administration of florfenicol,indicating that the pathological model was successful,the cumulative rate of 30% in low florfenicol group,0% in middle florfenicol group,0% in high florfenicol group,and 10% in treatment control group.After healing,no bacterial infection was found in the fish blood.The pathogenic bacterium Y.ruckeri was isolated from the liver and kidney of the experimental fish except negative control group,causing no death.The results showed that florfenicol had better effects in treatment of Siberia sturgeon infected by Y.ruckeri than chloramphenicol,and replaced chloramphenicol to treat bacterial diseases in fish.