| 梭梭(Haloxylon ammodendron)愈伤组织诱导及植株再生 |
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| 引用本文： | 施茜,孙振元,卢琦. 梭梭(Haloxylon ammodendron)愈伤组织诱导及植株再生[J]. 核农学报, 2005, 19(6): 441-446 |
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| 作者姓名： | 施茜 孙振元 卢琦 |
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| 作者单位： | 甘肃省治沙研究所,甘肃,武威,733000;中国林业科学研究院林业研究所/国家林业局林木培育重点实验室,北京,100091;中国防治荒漠化研究与发展中心/中国林业科学研究院,北京,100091 |
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| 基金项目： | 国家“948”项目(No.2001-03)，“十五”攻关(No.2000BA517A09)，国家转基因植物研究与产业化专项(JY03-B-28-02)，甘肃省科学事业费(QS031-C31-03) |
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| 摘 要： | 以梭梭(Haloxylon ammodendron)无菌苗的茎尖、子叶、下胚轴为材料进行愈伤组织诱导,培养基中单独添加1~3mg/L的2,4-D或与0.5mg/L的6-BA配合使用,均能成功诱导出愈伤组织,但在愈伤组织诱导起始阶段,用含2,4-D 2.0mg/L、BA 0.5mg/L的培养基效果较好;在2,4-D1.0mg/L、BA 0.5mg/L的分化培养基上经过两次连续继代培养后,在含BA 0.5mg/LI、AA 1.0mg/L的培养基上愈伤组织生长状态最好,并从茎尖诱导的愈伤组织上获得了再生植株。
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| 关 键 词： | 梭梭 组织培养 胚性愈伤 植株再生 |
| 文章编号： | 1000-8551(2005)06-441-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2005-07-11 |
CALLUS INDUCTION AND PLANT REGENERATION IN Haloxylon ammodendron |
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| SHI Qian,SUN Zhen-Yuan,LU Qi. CALLUS INDUCTION AND PLANT REGENERATION IN Haloxylon ammodendron[J]. Acta Agriculturae Nucleatae Sinica, 2005, 19(6): 441-446 |
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| Authors: | SHI Qian SUN Zhen-Yuan LU Qi |
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| Affiliation: | SHI Qian~1 SUN Zhen-Yuan~2 LU Qi~3 |
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| Abstract: | Protocols for in vitro plant regeneration via callus devised from shoot tip were established in an important desert plant,Haloxylon ammodendron.Callus induction has been achieved from cotyledons,hypocotyls and shoot tip explants.The highest frequency of callus induction and greatest amount of callus proliferation occurred on MS medium supplemented with 2.0mg/L 2,4-D combined with 0.5mg/L BA.Followed two generations of subcultures on MS medium supplemented with 1.0mg/L 2,4-D and 0.5mg/L BA.The highest frequency of embryogenic callus induction was achieved on MS medium supplemented with 1.0mg/L IAA combined with 0.5mg/L BA.At the same time,in vitro plant regeneration occurred from callus devised via shoot tip explants. |
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| Keywords: | Haloxylon ammodendren tissue culture embryogenic callus plant regeneration |
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