Asymmetric segregation of polarized antigen on B cell division shapes presentation capacity |
| |
Authors: | Thaunat Olivier Granja Aitor G Barral Patricia Filby Andrew Montaner Beatriz Collinson Lucy Martinez-Martin Nuria Harwood Naomi E Bruckbauer Andreas Batista Facundo D |
| |
Affiliation: | Lymphocyte Interaction Laboratory, London Research Institute, Cancer Research UK, 44 Lincoln's Inn Fields, London, UK. |
| |
Abstract: | During the activation of humoral immune responses, B cells acquire antigen for subsequent presentation to cognate T cells. Here we show that after mouse B cells accumulate antigen, it is maintained in a polarized distribution for extended periods in vivo. Using high-throughput imaging flow cytometry, we observed that this polarization is preserved during B cell division, promoting asymmetric antigen segregation among progeny. Antigen inheritance correlates with the ability of progeny to activate T cells: Daughter cells receiving larger antigen stores exhibit a prolonged capacity to present antigen, which renders them more effective in competing for T cell help. The generation of progeny with differential capacities for antigen presentation may have implications for somatic hypermutation and class switching during affinity maturation and as B cells commit to effector cell fates. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|