Plant regeneration from protoplasts of Nicotiana alata ‘Crimson Bedder’ for somatic hybridisation studies |
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| Authors: | SF Berry G Patnaik EC Cocking D Pental |
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| Institution: | Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, Nottingham, NG7 2RD Gt. Britain |
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| Abstract: | Protoplasts could be isolated readily from mesophyll and suspension cells of Nicotiana alata cultivar ‘Crimson Bedder’ and could be cultured in liquid media to yield small colonies. Suspension culture protoplasts exhibited a higher plating efficiency, and colonies regenerated from mesophyll protoplasts possessed enhanced plant regeneration capacity. These protoplast systems could be used for somatic hybridisation assessment with Petunia using a simple procedure for manual isolation and culture of heterokaryons. |
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| Keywords: | In vitro protoplasts regeneration somatic hybridisation |
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