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Molecular identification of Chilli leaf curl India virus along with betasatellite molecule causing leaf curl disease of menthol mint (Mentha arvensis var. Kosi) in India
Authors:Sana Tabanda Saeed  B. Kumar  A. K. Shasany  A. Samad
Affiliation:1.Plant Pathology Department,CSIR-Central Institute of Medicinal and Aromatic Plants,Lucknow,India;2.Genetics and Plant Breeding Division,CSIR-Central Institute of Medicinal and Aromatic Plants,Lucknow,India;3.Department of Biotechnology,CSIR-Central Institute of Medicinal and Aromatic Plants,Lucknow,India
Abstract:Whitefly-transmitted begomovirus induces leaf curling and stunting of Mentha arvensis (Kosi) and is responsible for heavy economic losses in and around Lucknow, India. The complete genome was amplified, sequenced and assembled as 2759 bp long, with 1376 bp betasatellite molecule. Because no DNA-B amplicon was detected, the virus was classified as “Old World begomovirus” with the characteristic seven ORFs; two ORFs (AV1, AV2) in the V-sense and five ORFs (AC1–AC5) in the C-sense. BLASTn and phylogenetic analysis showed highest similarity (98%) with chilli leaf curl India virus (ChiLCINV). This is the first report of ChiLCINV infection in M. arvensis (Kosi) from India.
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