Castanea sativa plantlets proliferated from axillary buds cultivated in vitro |
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Authors: | AM Vieitez ML Vieitez |
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Institution: | 1. Plant Physiology, C.S.I.C., Apartado 122, Santiago de Compostela, Spain;2. Department of Plant Physiology, Faculty of Biology, University of Santiago de Compostela Spain |
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Abstract: | Chestnut plants were proliferated in vitro from axillary buds of juvenile shoots. N6-Benzyl-aminopurine (BAP) at 0.1?0.5 mg l?1 was optimal for shoot multiplication. The important role played by the macronutrient formula on shoot multiplication, and especially on the rooting-stage, is emphasized. The MS ( NO3) macronutrients gave the best rooting percentage as well as the highest number of roots per rooted shoot. In these experiments, shoots remained in the 3 mg l?1 indole-3-butyric acid (IBA) medium for 12 days, after which they were transferred to an auxin-free medium where roots developed fully. Optimum rooting was achieved by immersing the 1 cm basal end of shoots in concentrated IBA solutions (0.5?1 mg ml?1) for periods ranging from 2 to 15 min. |
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