菊花2个品种高效再生体系的建立

以地被菊花2个品种‘铺地金’和‘北林红’的叶片为外植体,以MS为基本培养基,以不同浓度配比的6-BA,NAA为生长调节物质进行离体培养,直接分化出不定芽获得再生植株.试验表明:‘铺地金’叶片外植体再生的最适培养基为MS 6-BA 2.0 mg/L NAA 1.0 mg/L,再生率高达96%,其叶柄在此培养基上的再生率为90%;‘北林红’再生的最适宜培养基为MS 6-BA 1 mg/L NAA 0.5 mg/L,再生率为96%,但其茎段和叶柄在此培养基上不能高效再生.‘铺地金’腋芽增殖的最佳培养基为MS 6-BA 0.5 mg/L NAA 0.01 mg/L,每腋芽外植体平均增殖不定芽数达到5.5个.‘北林红’腋芽增殖的最佳培养基为MS 6-BA 0.5 mg/L NAA 0.05mg/L和MS 6-BA 0.5mg/L NAA 0.01mg/L,腋芽外植体平均增殖不定芽数达到6.3个和5.6个,二者之间的差异不显著.

Establishment of Efficient Propagation System for 2 Chrysanthemum Cultivars

Tissue culture experiment was conducted for 2 chrysanthemum cultivars 'pudijin' and 'beilinhong' to acquire plantlets by adventitious bud differentiation from leaf explant on MS medium by applying different concentrations and proportions of 6-BA and NAA plant growth regulators.The results showed that MS 6-BA 2.0 mg/L NAA 1.0 mg/L was the optimal leaf propagation medium for 'Pudijin' cultivar,on which the leaf proliferation rate was 96%,while the petiole proliferation rate was 90%.The optimal leaf propagation medium for 'Beilinhong' cultivar was MS 6-BA 1 mg/L NAA 0.5 mg/L,on which the proliferation rate was up to 96%,but other explants of the cultivar such as petiole or stem could not be propagated on this medium.The optimal axillary bud propagation medium for 'Pudijin' cultivar was MS 6-BA 0.5 mg/L NAA0.01 mg/L,by which the mean number of adventitious bud propagated from each axillary bud reached 5.5.MS 6-BA 0.5 mg/L NAA 0.05 mg/L and MS 6-BA 0.5 mg/L NAA 0.01 mg/L were the optimal propagation media for axillary bud explant of 'Beilinhong' cultivar,on which the average numbers of 6.3 and 5.6 adventitious buds could be obtained respectively from each axillary bud,while there was no significant difference between the number of adventitious buds obtained on the 2 media.