| 牛MyoD1基因遗传变异及其对胴体性状的影响 |
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| 引用本文： | 黄萌,许尚忠,昝林森,高雪,陈金宝. 牛MyoD1基因遗传变异及其对胴体性状的影响[J]. 中国畜牧兽医, 2007, 34(9): 40-43 |
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| 作者姓名： | 黄萌 许尚忠 昝林森 高雪 陈金宝 |
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| 作者单位： | 1. 西北农林科技大学动物科技学院，杨凌712100; 2. 中国农业科学院北京畜牧兽医研究所，北京100094 |
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| 基金项目： | 国家科技支撑计划重大项目;国家高技术研究发展计划(863计划) |
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| 摘 要： | 运用DNA池快速测序法寻找牛MyoD1基因SNPs，筛查到1个新的多态位点C1867T，该位点C-T突变引起甘氨酸/丝氨酸的转变。运用PCR RFLP法验证并分析该位点在不同群体间的多态性, 并与相应牛群体的胴体性状进行关联分析。结果显示，中国西门塔尔牛、鲁西牛群体的C1867T位点的突变达到了Hardy-Weinberg 平衡状态，而秦川牛未达到平衡状态。不同基因型对肉牛的背膘厚、大腿肉厚的影响差异极显著或显著(P<0.01或P<0.05)，对日增重、屠宰率、肌间脂肪、大理石花纹、嫩度的影响差异不显著。
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| 关 键 词： | 牛 MyoD1基因 多态性 基因效应 |
| 文章编号： | 1671-7236（2007）09-0040-04 |
| 修稿时间： | 2007-04-23 |
Polymorphism with Exon 3 of the Cattle MyoD1 Gene and Their Frequencies in Carcass Traits |
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| HUANG Meng,XU Shang-zhong,ZAN Lin-sen,GAO Xue,CHEN Jin-bao. Polymorphism with Exon 3 of the Cattle MyoD1 Gene and Their Frequencies in Carcass Traits[J]. China Animal Husbandry & Veterinary Medicine, 2007, 34(9): 40-43 |
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| Authors: | HUANG Meng XU Shang-zhong ZAN Lin-sen GAO Xue CHEN Jin-bao |
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| Affiliation: | 1. Animal Science-technology College, Northwest Agriculture and Forestry University, Yangling 712100, China；2. Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing 100094, China |
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| Abstract: | Based on gene pooling and sequencing,a new SNPs was found on the bovine MyoD1 gene,its transition C-T leads to a reaplacement of glicine with serine.Polymorphism and relationship with the carcass traits was analysised by PCR-RFLP in different populations.The transition of this locus fit in with Hardy-Weinberg equilibrium in Chinese-simmental cattle and Luxi cattle,but not in Qinchuan cattle.Statistical analysis revealed higher value in back fat and thickness of thigh(P<0.05),not in average daily growth,yield of carcass,intramuscular fat,marbling and yenderness. |
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| Keywords: | cattle MyoD1 gene polymorphism genetic effect |
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