橄榄苦苷对D-半乳糖致衰老小鼠衰老相关生化指标的影响

为了研究橄榄苦苷对D-半乳糖致衰老小鼠的抗衰老作用及其机制,将40只健康小鼠随机分成正常组、衰老模型组、橄榄苦苷组和维生素E组,采用D-半乳糖建立亚急性衰老小鼠模型,测定每组小鼠的脏器指数,比色法检测心、肝和脑组织中超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、及丙二醛(MDA)含量,PCR-ELISA检测心、肝和脑组织端粒酶活性,ELISA检测小鼠血清中Ig G、Ig M和补体C3、C4含量,放射免疫法测定血清中IL-2含量。结果显示,与模型组比较,橄榄苦苷能增加小鼠脏器指数(P0.05);提高SOD、GSH-Px活性,降低MDA含量(P0.05,P0.01);提高心、肝和脑端粒酶活性、血清中Ig G、Ig M和补体C3、C4及IL-2的含量(P0.05,P0.01),上述衰老相关生化指标基本恢复到正常水平,且与维生素E的治疗结果无显著差异(P0.05)。表明橄榄苦苷能通过拮抗自由基损伤,增强衰老小鼠心、肝和脑组织端粒酶活性和机体免疫功能发挥抗衰老作用。

Effect of oleuropein on the aging related biochemical indexes in aging mice induced by D-galactose

An experiment was conducted to explore the anti-aging effect of oleuropein on aging mice induced by D-galactose and relevant mechanisms. Forty mice were randomly divided into normal control group, aging model group, oleuropein treated group, and vitamin E control group. Aging mice models were developed using D-galactose. The viscera index was determined. Spectrophotometry was used to detect the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the heart, liver and brain of the mice. PCR-ELISA was used to measured telomerase activity in the heart, liver and brain. The levels of serum IgG, IgM and complement C3, C4 and interleukin-2 (IL-2) were detected by ELISA and radioimmunoassay, respectively. The results showed that oleuropein could significantly increase the viscera index, elevate the activities of SOD and GSH-Px, decrease the content of MDA, and increase telomerase activity in the heart, liver and brain of the mice and the levels of serum IgG, IgM and complement C3, C4 and IL-2 (P<0.05, P <0.01). The above aging related biochemical indexes returned the level close to the normal after oleuropein treatment, and no statistical difference was found in the treatment efficacy compared with those in vitamin E control group (P >0.05). The study suggested that oleuropein could delay aging, which may be related to antagonizing free radical injury and enhancing the telomerase activity in the heart, liver and brain, and immune function of D-galactoce- induced aging mice.