金黄色葡萄球菌荚膜多糖的提取及其多糖含量的测定

为提取纯化金黄色葡萄球菌荚膜多糖并测定多糖含量.采用超声波破碎菌液,用CTAB结合其中的多糖、CaCl2解离、乙醇沉淀等化学方法提取纯化金黄色葡萄球菌荚膜多糖,利用苯酚-硫酸法测定其含量.结果显示,得到荚膜多糖0.31 g,含量为65.06%.结果表明,成功提取金黄色葡萄球菌荚膜多糖,并对其含量进行了测定,初步确定了粗提金黄色葡萄球茵荚膜多糖的方法.

Extraction and determination of Staphylococcus aureus capsular polysaccharide

The objective of this experiment was to extracted and purified capsular polysaccharide from Staphylococcus aureus,and detected it is contents. Supersonic wave was used to crushed bacteria liquid, CTAB was used to combine capsular polysaccharide, CaCl2 decollement and ethanol precipitation of chemical method was used to extracte and purify capsular polysaccharide, phenol-vitriolic colorimetry was used to detecte its contents.The result showed that obtained capsular polysaccharide 0.31g,its contents was 65.06%. The result indicated that this experiment successed to extract capsular polysaccharide,and determined its contents, preliminary determined methods of crudely extracted capsular polysaccharide of Staphylococcus aureus.