单细胞凝胶电泳技术检测蜘蛛血细胞DNA损伤研究

以体外染毒法对星豹蛛雌、雄成蛛进行过氧化氢和甲醛染毒处理,采用单细胞凝胶电泳技术检测蜘蛛血细胞DNA损伤效应,用CASP软件分析彗星图像,并计算受损伤细胞率、彗星尾长和Olive尾矩.结果表明,不同浓度的过氧化氢能引起蜘蛛血细胞DNA断裂损伤,且损伤程度与浓度之间有明显的剂量-效应关系;不同浓度的甲醛能引起蜘蛛血细胞DNA损伤,其中在10ümol/L时引起DNA断裂,在25ümol/L、40ümol/L、55 ümol/L时引起DNA交联,而在70ümol/L时引起血细胞凋亡.

Study on DNA damages of hemocytes in spider with single cell gel electrophoresis

To study and evaluate genotoxicity of H2O2 and formaldehyde in the spider, pardosa astrigera, the adult of female and male spiders were treated with H2O2 at concentrations of 50,75,100,125,150µM and formaldehyde at 10,25,40,55,70µM, respectively. DNA damage of hemocytes was detected by the comet assay. CASP software was used to analyze the comet pictures. The results show that the percentage of comet, the percentage of tail length, and the tail moment of the H2O2 tested groups were significantly different from those of the control group (P<0.05). A statistically significant dose-dependent increase in DNA damage was observed. Formaldehyde induces DNA strand breaks at 10µM, but crosslinks at 25,40,55µM, and apoptosises at 70µM in hemocytes cells of spiders.