| 崂山奶山羊瘤胃微生物总DNA的提取方法优化 |
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| 引用本文： | 王利华,隋昶生. 崂山奶山羊瘤胃微生物总DNA的提取方法优化[J]. 勤云标准版测试, 2009, 29(5) |
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| 作者姓名： | 王利华 隋昶生 |
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| 作者单位： | 1. 青岛农业大学2. 青岛农业大学动物科学与技术学院 |
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| 摘 要： | 本试验对对瘤胃微生物总DNA提取方法进行了改进，将冻融法和CATB法相结合，可以将细菌、真菌和古细菌在内的各种微生物的细胞壁充分的粉碎，且DNA提取效果稳定，可以获得足够大的DNA片段（23kb），可以满足后续试验的需要
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| 关 键 词： | Total DNA freezing-thawing method CATB |
Extraction method optimizing of total DNA in rumen microorganism of Laoshan dariy |
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| Abstract: | The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB. Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus. DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research. It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA. |
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