| 农杆菌介导法将Bt基因转入大豆的研究 |
| |
| 引用本文： | 刘尚前,王罡,季静,王萍. 农杆菌介导法将Bt基因转入大豆的研究[J]. 大豆科学, 2007, 26(1): 103-106 |
| |
| 作者姓名： | 刘尚前 王罡 季静 王萍 |
| |
| 作者单位： | 河北北方学院农业科学系,宣化,075131;天津大学农业与生物工程学院,天津,300072;淮海工学院海洋学院,连云港,222005 |
| |
| 基金项目： | 本研究由“国家植物转基因技术研究开发与中试基地建设”专项课题（J99-B-001）资助 |
| |
| 摘 要： | 以6个品种大豆体细胞胚为受体，以Bt基因为目的基因，应用根癌农杆茵介导法对大豆进行了遗传转化，经体细胞胚萌发、生根及壮苗培养和卡那霉素选择培养，获得了完整的抗性再生苗，经PCR、PCR-Southern和点杂交分析鉴定，初步证明外源基因Bt已整合到大豆的基因组中。
|
| 关 键 词： | 大豆 体细胞胚 Bt基因 遗传转化 |
| 文章编号： | 1000-9841（2007）01-0103-04 |
| 修稿时间： | 2005-12-21 |
PUT BT GENE TRANSFER INTO SOMATIC VIA AGROBACTERIUM TUMEFACIEN |
| |
| LIU Shang-qian,WANG Gang,JI Jing,WANG Ping. PUT BT GENE TRANSFER INTO SOMATIC VIA AGROBACTERIUM TUMEFACIEN[J]. Soybean Science, 2007, 26(1): 103-106 |
| |
| Authors: | LIU Shang-qian WANG Gang JI Jing WANG Ping |
| |
| Affiliation: | 1. Department of Agriculture Sciences of Hebei North University, Xuanhua 075131 ; 2. Tianjin University of Agricultural and Biological Engineering, Tianjin 300072; 3. Huaihai Institute of Technology, Marine School ,Lianyungang 222005 |
| |
| Abstract: | Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens. The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR. These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. The results demonstrated that the target gene had been transformed into the soybean genome. |
| |
| Keywords: | Soybean Somatic embryos Bt gene Genetic transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
