NO合酶抑制剂 L-NAME和NO前体L-Arg对小鼠体内卵母细胞成熟的影响 |
| |
引用本文: | 卜淑敏,夏国良,王松波,汪建红. NO合酶抑制剂 L-NAME和NO前体L-Arg对小鼠体内卵母细胞成熟的影响[J]. 农业生物技术学报, 2003, 11(3): 291-294 |
| |
作者姓名: | 卜淑敏 夏国良 王松波 汪建红 |
| |
作者单位: | 1. 中国农业大学生物学院,北京,100094;山西大学生命科学与技术学院,太原,030006 2. 中国农业大学生物学院,北京,100094 3. 中国农业大学生物学院,北京,100094;新疆师范大学生命与环境科学学院,乌鲁木齐,830054 |
| |
基金项目: | 国家杰出青年基金资助(30025032). |
| |
摘 要: | 摘要: 采用腹腔单独注射L-NAME(左旋硝基精氨酸甲基酯)或联合注射L-NAME和L-Arg, 取出卵母细胞体外观察,研究了NO对小鼠卵母细胞体内成熟的影响。结果表明,(1)单独注射5、10、20 mg / kg体重L-NAME均能显著抑制卵母细胞第一极体的释放(P <0.01),尤以10 mg / kg 体重L-NAME组作用最为明显, 各剂量组对生发泡的破裂均无影响(P >0.05);(2)10 mg / kg 体重L-NAME对卵母细胞成熟的抑制作用能被同时注射的100 mg / kg 或200 mg /kg 体重L-Arg逆转,其中以200 mg / kg体重作用最为明显;(3)L-NAME对卵母细胞畸形率的影响和培养时间有关,而对卵母细胞的存活率没有统计学影响。实验结果证明NO参与了小鼠卵母细胞的体内成熟调控。
|
关 键 词: | 关键词:NO 卵母细胞 减数分裂 NO合酶 |
修稿时间: | 2002-06-18 |
Effects of L-NAME and L-Arg on Meiotic Maturation of Mouse Oocyte in Vivo |
| |
Abstract: | Abstract: The effect of nitric oxide (NO)on meiotic maturation of mouse oocyte in vivo was investigated by injecting L-NAME alone or co-injecting L-NAME and L-Arg intraperitoneally. The results showed that: (1) 5, 10 and 20 mg/kg bw (body weight) of L-NAME inhibited significantly the extrusion of first polar body of mouse oocyte(P <0.01), especially 10 mg/kg bw. But they all had little impact on germinal vesicle breakdown (P >0.05). (2) The inhibitory effect of L-NAME (10 mg/kg bw) on the extrusion of first polar body of mouse oocyte could be reversed by 100 mg/kg bw or 200 mg/kg bw L-Arg.(3) The abnormality caused by L-NAME was related to the culture time, but L-NAME had no significant effect on viability of mouse oocytes. It could be concluded that NO was involved in regulation of mouse oocyte meiotic maturation in vivo. |
| |
Keywords: | NO |
本文献已被 万方数据 等数据库收录! |
| 点击此处可从《农业生物技术学报》浏览原始摘要信息 |
|