| 类番茄茄茎段原生质体再生成株的研究 |
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| 引用本文： | 张长远,吴定华. 类番茄茄茎段原生质体再生成株的研究[J]. 江西农业大学学报, 2001, 23(2): 200-202 |
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| 作者姓名： | 张长远 吴定华 |
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| 作者单位： | 1. 广东省农业科学院蔬菜研究所,
2. 华南农业大学园艺系, |
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| 基金项目： | 国家九五重点科技攻关项目资助! (96 - 0 0 2 - 0 2 - 2 1) |
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| 摘 要： | 利用类番茄茄 (SolamumlycopersicoidesDun .)无菌苗幼嫩茎段酶解获得大量原生质体 (个 ) (2× 10 6/g) ,将原生质体密度稀释至 1× 10 5/mL于HMA培养基中培养 ,则 10d左右出现小细胞团 ,38～ 40d形成肉眼可见的小愈伤组织 (1～ 1.5mm) ,转移至MC增殖培养基 2周后 ,再转移到 15 #分化培养基诱导出芽 ,切取芽体转入5 0P培养基诱发根原基 ,再转入 5 0 #发根培养基形成发达根系 ,成为再生植株 ,整个再生周期 80～ 90d ,再生植株移植至土壤中均能正常生长、开花
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| 关 键 词： | 类番茄 茄茎段 原生质体 再生植株 番茄 体细胞杂交 |
| 文章编号： | 1000－2286(2001)02－0200-03 |
| 修稿时间： | 2000-12-14 |
Plant Regeneration from Stem-Derived Protoplasts of Solanum lycopersicoides |
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| ZHANG Chang-yuan,WU Ding-hua. Plant Regeneration from Stem-Derived Protoplasts of Solanum lycopersicoides[J]. Acta Agriculturae Universitis Jiangxiensis, 2001, 23(2): 200-202 |
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| Authors: | ZHANG Chang-yuan WU Ding-hua |
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| Affiliation: | ZHANG Chang-yuan 1,WU Ding-hua 2 |
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| Abstract: | A procedure for protoplast isolation, culture and plant regeneration has been developed for solanum lycopersicoides. Stem－protoplasts were diluted to 1×105/mL and cultured in HMA medium, cell colonies (20～30 cells) appeared after 10 days, micro calli (1～1.5mm) formed after 38～40 days from initially culturing. The micro calli were transferred to MC greenig medium for 2 weeks. The green calli were transferred to 15# medium for shoot inducting. The shoots which excised from the callus were transferred to test tubes with 50p medium first for root promoting about 3～4 days and then transferred to 50# rooting medium. Plants regenerated after 80～90 days after initially culturing. All the plants which potted in soil could vigorously grow and blossom. |
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| Keywords: | Solanum lycopersicoides stem-derived protoplast plant regereration |
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