| 理化诱变提高鼠李糖乳杆菌产L-乳酸的研究 |
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| 引用本文： | 李元媛,高媛,蔡俊.理化诱变提高鼠李糖乳杆菌产L-乳酸的研究[J].湖北农业科学,2010,49(1). |
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| 作者姓名： | 李元媛 高媛 蔡俊 |
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| 作者单位： | 1. 湖北工业大学生物工程学院,武汉,430068
2. 湖北工业大学生物工程学院,武汉,430068;发酵工程省部共建教育部重点实验室,湖北工业大学,武汉,430068;湖北省工业微生物重点实验室,武汉,430068 |
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| 基金项目： | 湖北省教育厅攻关项目 |
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| 摘 要： | 以鼠李糖乳杆菌(Lactobacillus rhamnosus)为出发菌株,经紫外线、氯化锂复合诱变处理,再用溴甲酚紫平板及摇瓶复筛得到一株高产L-乳酸的正向突变株L3,平均发酵产量为58.65 g/L,比原菌株产量提高60.77%。
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| 关 键 词： | 鼠李糖乳杆菌 L-乳酸 诱变育种 |
Mutation Breeding of High-yield Strains of L-Lactic Acid |
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| LI Yuan-yuan,GAO Yuan,CAI Jun.Mutation Breeding of High-yield Strains of L-Lactic Acid[J].Hubei Agricultural Sciences,2010,49(1). |
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| Authors: | LI Yuan-yuan GAO Yuan CAI Jun |
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| Abstract: | In order to obtain a industrial strain with higher L-lactic acid yield,the original strain Lactobacillus rhamnosus was mutated by means of compound mutagenesis(UV and LiCl)and selected by bromocresol purple plate.The selected strain was studied by fermentation in shake flask.Then,a mutation strain L3 with a higher L-lactic acid production of 58.65g/L was obtained,L-lactic acid was enhanced by 60.77%compared with that of original strain. |
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| Keywords: | Lactobacillus rhamnosus L-lactic acid mutation breeding |
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