| Mapping a Novel Gene of Cold Tolerance at Booting Stage by Using Near-Isogenic Lines in japonica Rice |
| |
| 作者姓名： | SHEY Shi-quan ZENG Ya-wen LI Shen-chong WEN Guo-song Pu Xiao-ying |
| |
| 作者单位： | Biotechnology and Genetic Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China |
| |
| 摘 要： | Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines （NILs） of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats （SSR） distributed over 12 chromosomes were used to screen polymorphism between Towata （recurrent parent, RP） and near-isogenic line pool （NILP）, and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb（t）.
|
| 关 键 词： | 水稻 基因分析 抗寒型 基因序列 |
| 收稿时间： | 2004-12-30 |
| 修稿时间： | 2005-03-02 |
Mapping a Novel Gene of Cold Tolerance at Booting Stage by Using Near-Isogenic Lines in japonica Rice |
| |
| SHEY Shi-quan ZENG Ya-wen LI Shen-chong WEN Guo-song Pu Xiao-ying.Mapping a Novel Gene of Cold Tolerance at Booting Stage by Using Near-Isogenic Lines in japonica Rice[J].Rice science,2005,12(3):163-167. |
| |
| Authors: | SHEY;Shi-quan;ZENG;Ya-wen;LI;Shen-chong;WEN;Guo-song;Pu;Xiao-ying |
| |
| Abstract: | Genetic analysis showed that cold tolerance at booting stage of near-isogenic lines (NILs) of Kunmingxiaobaigu was controlled by a gene with large phenotypic variance. One hundred and sixty-four simple sequence repeats (SSR) distributed over 12 chromosomes were used to screen polymorphism between Towata (recurrent parent, RP) and near-isogenic line pool (NILP), and two SSR markers at the long arm of chromosome 5 showed polymorphism in comparison with RP genome. Of the two markers, RM31 was found possibly linked with the cold tolerance gene at booting stage through one-way ANOVA. Twelve SSR markers around RM31 were then used to detect polymorphism between RP and NIL, and only RM7452 had polymorphism. The gene of cold tolerance at booting stage was further mapped on chromosome 5 between RM7452 and RM31 with genetic distances of 4.8 cM and 8.0 cM, respectively. This gene explained 10.50% of phenotypic variance and 5.10% of phenotypic variance of fully filled grains, and was tentatively designated as Ctb(t). |
| |
| Keywords: | booting stage near-isogenic line simple sequence repeat genetic analysis cold tolerance gene |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
