In-situ enrichment and analysis of atrazine-degrading microbial communities using atrazine-containing porous beads |
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Authors: | Dhritiman Ghosh Velusamy Srinivasan Olli H. Tuovinen Aaron Peacock Mark Radosevich |
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Affiliation: | a Biosystems Engineering and Soil Science, University of Tennessee, 2506 E.J. Chapman Dr., Knoxville, TN 37996, United States b Plant Sciences Department, University of Tennessee, Knoxville, TN 37996, United States c Department of Microbiology, The Ohio State University, Columbus, OH 43210, United States d Chemical Engineering Department, University of Tulsa, Tulsa, OK 74104, United States |
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Abstract: | We examined the community composition of microbes that colonized atrazine-containing beads buried in agricultural soils that differed in atrazine treatment history. Bacterial abundance was 5-40-fold greater in atrazine-fortified beads. In beads containing 20 mg atrazine kg−1 buried in soil with a history of atrazine application (conditioned soil), the abundance of Actinobacteria increased approximately 80-fold whereas in control soil, Actinobacteria were enriched only 10-fold and the gamma-Proteobacteria and Planctomycetes increased by 60- and 25-fold, respectively. The gamma-Proteobacteria were enriched by 120- and 230-fold in beads containing 200 mg atrazine kg−1 in conditioned and control soil, respectively. The results demonstrate that BioSep® beads are a suitable matrix for recruiting a diverse subset of the bacterial community involved in atrazine degradation. |
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Keywords: | Soil Atrazine DGGE FISH Bacteria Community Enrichment BioSep® beads |
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