Evaluation of sample pre-treatment and potential contamination of soluble carbon in soil extracts during lyophilisation |
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Authors: | Filip Coppens Gonzague Alavoine Olivier Delfosse Sylvie Recous |
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Institution: | a INRA, UR1158 Agronomie Laon-Reims-Mons, Rue Fernand Christ, F-02000 Laon, France b Division Soil and Water Management, Department of Land Management and Economics, K.U. Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium c INRA, UMR614 Fractionnement des Agroressources et Environnement, CREA, 2 Esplanade Roland Garros, BP 224, F-51686 Reims Cedex 2, France |
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Abstract: | Lyophilisation of K2SO4 soil extracts has been proposed as a sample preparation technique before elemental analysis of carbon or nitrogen. However, previous measurements, based on wet oxidation or catalytic combustion, indicated that C measurements in lyophilised samples not always proved to be accurate. To determine whether the C analysis was affected by the lyophilisation process, an exploratory study was conducted to investigate the potential effects of the sample pre-treatment and of the lyophilisation process itself. This paper puts forward that the use of soil extracts, previously stored at −20 °C, may affect the recovery of salt in the samples and that contamination of the soluble carbon with exogenous C during lyophilisation is feasible. Therefore we recommend to use freshly prepared soil extracts for lyophilisation and always to include an internal standard among the unknown samples to account for a possible contamination. |
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Keywords: | Sample pre-treatment Freezing Soluble carbon Lyophilisation/lyophilization Contamination Freeze-drying |
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