| HAV表位抗原与HBV核心抗原融合基因的原核表达 |
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| 引用本文： | 张蕾,张玉静,万家余,韩烨.HAV表位抗原与HBV核心抗原融合基因的原核表达[J].吉林农业大学学报,2005,27(4):461-463. |
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| 作者姓名： | 张蕾 张玉静 万家余 韩烨 |
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| 作者单位： | 吉林大学农学部畜牧兽医学院,长春,130062;吉林大学农学部畜牧兽医学院,长春,130062;吉林大学农学部畜牧兽医学院,长春,130062;吉林大学农学部畜牧兽医学院,长春,130062 |
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| 基金项目： | 国家自然科学基金资助项目（C02030604） |
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| 摘 要： | 为探讨甲肝DNA疫苗的可行性，利用DNA重组技术将甲肝病毒（HAV）复合多表位抗原基因VPx插入到乙肝病毒核心抗原（HBcAg）基因中，得到CVPx融合基因。将CVPx克隆到原核表达载体pET-28a（＋）中，并在大肠杆菌中进行表达，表达的蛋白进行Western Blot及ELISA检测，结果表明：融合蛋白具有甲肝病毒表位抗原的反应原性。
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| 关 键 词： | HAV表位抗原 HBV核心抗原 E.coli表达 |
| 文章编号： | 1000-5684（2005）04-0461-03 |
| 收稿时间： | 2004-12-30 |
| 修稿时间： | 2004-12-30 |
E. Coli Expression of Fusion Gene of Epitopes Antigen of Hepatitis A Virus with Hepatitis B Core Antigen |
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| ZHANG Lei,ZHANG Yu-jing,WAN Jia-yu,HAN Ye.E. Coli Expression of Fusion Gene of Epitopes Antigen of Hepatitis A Virus with Hepatitis B Core Antigen[J].Journal of Jilin Agricultural University,2005,27(4):461-463. |
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| Authors: | ZHANG Lei ZHANG Yu-jing WAN Jia-yu HAN Ye |
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| Abstract: | In order to explore the possibility of HAV DNA vaccine, a multi-epitopes antigen gene VP_X of HAV was fused with HBcAg gene and CVP_X gene was constructed. CVP_X was sub-cloned into expression vector pET-28a(+) and expressed in E.coli. The products were analyzed by Western Blot and ELISA, which confirmed the fused protein had high antigenicity of HAV. This research is the foundation for the further study of HAV DNA vaccine. |
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| Keywords: | epitopes antigen of hepatitis a virus hepatitis b core antigen E coli expression |
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