|
|||||
|
|
| 荸荠基因组DNA的提取及RAPD反应体系的建立 | |
| 引用本文： | 江文,陈丽娟,李杨瑞,杨丽涛.荸荠基因组DNA的提取及RAPD反应体系的建立[J].广西农业科学,2009,40(3):229-232. |
| 作者姓名： | 江文 陈丽娟 李杨瑞 杨丽涛 |
| 作者单位： | 1. 广西大学农学院,南宁,530005;广西农业科学院生物技术研究所,南宁,530007;广西农业科学院广西作物遗传改良生物技术重点开放实验室,南宁,530007 2. 广西农业科学院生物技术研究所,南宁,530007 3. 广西大学农学院,南宁,530005;广西农业科学院广西作物遗传改良生物技术重点开放实验室,南宁,530007 4. 广西大学农学院,南宁,530005 |
| 摘 要： | 以荸荠叶状茎为试验材料，采用改良的SDS法提取其基因组DNA，并对其RAPD反应体系进行优化，建立了荸荠的RAPD—PCR优化反应体系和程序。结果表明，提取的基因组DNA纯度和完整性较好，OD260/OD280值在1．8～2．0之间，DNA无降解现象，完全可以满足RAPD—PCR扩增要求。建立了荸荠RAPD反应体系：总体积为25μl，各有关成分的最佳浓度分别为25mmol／L Mg^2＋，1．0UTaqDNA聚合酶，0．2mmol／L dNTPs，1μmol／μl引物，1．5ng／μl DNA模板。PCR反应程序为：94％预变性3min；94℃变性1min，37℃退火30s，72℃延伸60s，40个循环；最后72％延伸10min。 |
| 关 键 词： | 荸荠 基因组DNA 提取 RAPD 反应体系 |
Extraction of genomic DNA and establishment of RAPD reaction system for water chestnut(Eleocharis tuberose Roem et Schult) |
|
| JIANG Wen,CHEN Li-juan,LI Yang-rui,YANG Li-tao.Extraction of genomic DNA and establishment of RAPD reaction system for water chestnut(Eleocharis tuberose Roem et Schult)[J].Guangxi Agricultural Sciences,2009,40(3):229-232. | |
| Authors: | JIANG Wen CHEN Li-juan LI Yang-rui YANG Li-tao |
| Institution: | 1 Agricultural College;Guangxi University;Nanning 530005;China;2 Biotechnology Research Institute;Guangxi Academy of Agricultural Sciences;Nanning 530007;3 Guangxi Crop Genetic Improvement and Biotechnology Laboratory;China |
| Abstract: | The young phylloclade of water chestnut was used as experimental material,and the improved SDS method was used to extract the genomic DNA of young phylloclade successfully.The results showed that the extracted genomic DNA was pure and integral,and the ratio of OD260/OD280 was from 1.8 to 2.0,and the degradation of extracted genomic DNA was not found.Therefore,the extracted genomic DNA was suitable for RAPD-PCR reaction.The conditions for RAPD-PCR were optimized,and the best RAPD reaction system was establis... |
| Keywords: | RAPD water chestnut (Eleocharis tuberose Roem et Schuh) genomie DNA extraction RAPD reaction system |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |