| 正交实验法优化转基因玉米中植酸酶的提取工艺 |
| |
| 引用本文： | 刘 娜,柳鸿敏,于 壮,张欣芳,盖春宇,邢珍娟,陈奕冰,李 静,张 明.正交实验法优化转基因玉米中植酸酶的提取工艺[J].玉米科学,2011,19(4):142-144. |
| |
| 作者姓名： | 刘 娜 柳鸿敏 于 壮 张欣芳 盖春宇 邢珍娟 陈奕冰 李 静 张 明 |
| |
| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130033
2. 吉林大学公共卫生学院,长春,130021 |
| |
| 基金项目： | 转基因生物新品种培育重大专项课题(2008ZX08012-04、 2008ZX08011-003) |
| |
| 摘 要： | 采用正交实验法研究pH值、料液比和提取时间等因素对酶提取效率的影响,探索超声提取转基因玉米中植酸酶最佳提取条件。结果显示,最佳提取条件为提取液pH值5.5,料液比为1∶4,提取时为15 min。
|
| 关 键 词： | 转基因玉米 植酸酶 正交实验 |
| 收稿时间： | 2010/9/10 0:00:00 |
Optimization of the Extract Technology on the Phytase from Transgenic Corn by Orthogonal Experiment |
| |
| LIU N,LIU Hong-min,LI Jing,ZHANG Ming,et al..Optimization of the Extract Technology on the Phytase from Transgenic Corn by Orthogonal Experiment[J].Journal of Maize Sciences,2011,19(4):142-144. |
| |
| Authors: | LIU N LIU Hong-min LI Jing ZHANG Ming |
| |
| Institution: | LIU Na 1,LIU Hong-min 2,LI Jing 2,ZHANG Ming 1,et al.(1.Biotechnology Research Center,Jilin Academy of Agricultural Sciences,Changchun 130033,2.School of Public Health,Jilin University,Changchun 130021,China) |
| |
| Abstract: | Orthogonal test method was used to study the ultrasonic extraction conditions of the phytase from transgenic corn. It was found that the content of the phytase extracted was the highest under the conditions of ultrasonic extraction for 15min, using pH 5.50 buffer liquid at a liquid-solid ratio of 4 to1, which guaranteed for the evaluation of the transgenic corn. |
| |
| Keywords: | Transgenic corn Phytase Orthogonal test |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《玉米科学》浏览原始摘要信息 |
| 点击此处可从《玉米科学》下载免费的PDF全文 |
|
