转基因油菜的遗传学分析

本文从以下几方面对油菜转Bt基因植株进行了研究:R_1、R_2代的Km抗性;R_0、R_1代的Southern杂交;R_0代抗虫试验以及R_0代的酯酶活力测定.实验结果分别是:经Km抗性试验,芥菜型油菜R_1代在12个无性系中有10个无性系符合孟德尔单基因分离的遗传规律,R_2代在7个无性系中有6个符合这一规律;分子杂交证明部分油菜植株的基因组中存在Bt基因的同源顺序,表明Bt基因已整合进植物基因组;抗虫试验的结果是芥菜型油菜叶片饲喂幼虫10d后各处理组活虫体重与对照比,有5个无性系存在显著差异,其中最高虫试死亡率是77.7%,甘蓝型油菜则有9个无性系存在显著性差异,最高死亡率是70%;酯酶活力测定的结果表明外源基因没有影响酯酶活力.

GENETIC ANALYSIS OF TRANSGENIC RAPE (Brassicajuncea and B.napus)

In this paper we studied the kanamycin resistance of R1and R2generations, Southern blotting annlysis of R0and R1gencrations, insect resistance of R0generation and the measurment of the total activity of non-specific esterases. The results of Km resistance were ten clones of R1and six clones of R2had the segregation rates of 3: 1. Southern blotting analysis confirmed that the genomes of some plants contained the homo geneous sequence of the Bt gene. The mortality of the larvae up to 77.7% in test for insecticidal activity of B. juncea and 5 clones were high difference in the larvae weight after ten day's feeding. In the same way, the results of B. napus'up to 70% and 9 clones' were high difference. Activities of the non-specific esterases in transgenic plants were no differ-ence with the control, which indicated that the foreign gene did not influence the activity of non-specific esterases.