Assessment of factors affectingin vitro shoot regeneration from axillary bud explant ofCamptotheca acuminata

Axillary buds from 3-yr.-old seedlings ofCamptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concentrations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1 mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8. Foundation item: The research was supported by the Key Project of Chinese Ministry of Education (03061) and Supported by Application Fund of Agricultural Research Production (03EFN216700297). Biography: Wang Hui-mei (1973-), female, Ph.D., Lecturer in Northeast Forestry University, Harbin 150040, P.R. China. Responsible editor: Zhu Hong