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Screening of bacterial DNA in bile sampled from healthy dogs and dogs suffering from liver- or gallbladder-associated disease |
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| Authors: | Sakurako NEO Iyo TAKEMURA-UCHIYAMA Jumpei UCHIYAMA Hironobu MURAKAMI Ayaka SHIMA Hideki KAYANUMA Taiki YOKOYAMA Satoshi TAKAGI Eiichi KANAI Masaharu HISASUE |
| Affiliation: | 1)School of Veterinary Medicine, Azabu University, Kanagawa, Japan;2)Department of Bacteriology, Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, Japan;3)Anicom Specialty Medical Institute Inc., Tokyo, Japan |
| Abstract: | Although the biliary system is generally aseptic, gallbladder microbiota has been reported in humans and some animals apart from dogs. We screened and analyzed the bacterial deoxyribonucleic acid in canine gallbladders using bile sampled from 7 healthy dogs and 52 dogs with liver- or gallbladder-associated disease. PCR screening detected bacteria in 17.3% of diseased dogs (9/52) and none in healthy dogs. Microbiota analysis of PCR-positive samples showed that the microbial diversity differed between liver- and gallbladder-associated disease groups. Thus, a specific bacterial community appears to occur at a certain frequency in the bile of diseased dogs. |
| Keywords: | bile bile microbiota gallbladder hospitalized dog laboratory dog |