我国大陆地区首例鹦鹉喙羽病的诊断及其致病病毒C1基因的序列分析

2008年7月末,我国青岛即墨地区患病的虎皮鹦鹉幼雏出现体重下降、脱羽和羽毛变形萎缩、鸟喙变形及胸腺结构变异等症状,怀疑为鹦鹉喙羽病(PBFD)。利用PCR对濒死鹦鹉进行鹦鹉喙羽病病毒(PBFDV)的检测,并对扩增的C1基因进行了测序与分析。结果表明,有两只检测为PBFDV阳性,B last分析发现,QD-CN08株与GenBank中已发表的PBFDV分离株C1基因同源性为82%~93%,进化树分析表明与日本的毒株有比较近的亲缘关系。结合临床症状、流行病学特征和实验室诊断,确诊为鹦鹉喙羽病,此病为中国大陆首次报道。

Diagnosis of the first psittacine beak and feather disease in the mainland of China and sequence analysis of viral C1 gene

At the end of July in 2008,an acute disease occurred in Melopsittacus undulatus in Qingdao,China.Clinical symptoms generally progressed to weight loss,feather dystrophy and loss and,in some severe cases,deformities of the beak and claws,and thymus damage.The diseased animals from two farms were detected for psittacine beak and feather disease virus(PBFDV) by PCR.Furthermore,the C1 gene was sequenced and analyzed.The result showed that two of the four samples were PBFDV positive.The C1 gene sequence was from 82% to 93% identical to the published nucleotide sequences of PBFDV in GenBank.Phylogenetic tree analysis showed that QD-CN 08 strain had relatively close genetic relationship with Japan′s PBFDV strains.Combined with clinical symptoms,epidemiological characteristics and laboratory diagnosis,the outbreak of psittacine beak and feather disease was confirmed.This disease is first reported in mainland of china.