| 植物原生质体研究概况 |
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| 引用本文： | 张颖,张微微,王芳. 植物原生质体研究概况[J]. 安徽农业科学, 2010, 38(8): 3941-3943. DOI: 10.3969/j.issn.0517-6611.2010.08.037 |
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| 作者姓名： | 张颖 张微微 王芳 |
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| 作者单位： | 江苏宿迁学院,江苏宿迁,223800;上海交通大学,上海,201101 |
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| 摘 要： | 植物原生质体分离、培养及植株再生技术是进行植物育种、基因工程、遗传理论及细胞生理生化特性研究的平台,具有重要的研究意义。该研究对影响原生质体分离和培养的重要因素包括酶、酶解时间、渗透压稳定剂和培养方法等进行了综述,同时提出植物原生质体再生植株遗传性状稳定性、变异规律等问题,为今后植物原生质体分离和培养研究提供借鉴。
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| 关 键 词： | 原生质体 分离 培养 意义 影响因素 |
Advances in Study on Plants' Protoplast |
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| ZHANG Ying et al. Advances in Study on Plants' Protoplast[J]. Journal of Anhui Agricultural Sciences, 2010, 38(8): 3941-3943. DOI: 10.3969/j.issn.0517-6611.2010.08.037 |
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| Authors: | ZHANG Ying et al |
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| Affiliation: | Jiangsu Suqian College;Suqian;Jiangsu 223800 |
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| Abstract: | Isolation and culture of plant protoplast and plant regeneration technology supply a platform for plantbreeding,biotechnology,genetic engineering and cell physiological and biochemical characteristics research,with important research significance.The important influencing factors of the isolation and culture of protoplast were summarized,including enzymes,different digestion time,osmotic pressure stabilizer and culture methods.At the same time,the problems of the stability and variation laws of regenerated ... |
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| Keywords: | Protoplast Isolation Culture Significance Influencing factors |
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