| 利用替代寄主增殖SfaMNPV的研究 |
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| 引用本文： | 惠丰立,王小立,刘征,彭建新. 利用替代寄主增殖SfaMNPV的研究[J]. 河南农业科学, 2003, 0(5): 28-29 |
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| 作者姓名： | 惠丰立 王小立 刘征 彭建新 |
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| 作者单位： | 1. 南阳师范学院生物系,河南,南阳,473061
2. 华中师范大学 |
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| 基金项目： | 河南省自然科学基金 (0 0 4 0 12 4 0 0 )，河南省教育厅自然科学基础项目 (98180 0 2 0 )资助 |
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| 摘 要： | 利用替代寄主棉铃虫增殖SfaMNPV时 ,不同的接种浓度、虫龄及饲养温度对棉铃虫增殖病毒都有一定的影响。用 1× 1 0 7PIB/ml接种浓度感染三龄幼虫 ,幼虫饲养温度为 2 5℃时 ,获得的病毒产量最高 ,为每克虫尸含多角体 78.34亿。
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| 关 键 词： | 替代寄主 棉铃虫 SfaMNPV 增殖 |
Studies on Multiplication of SfaMNPV by Using Substitutive Host |
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| HUI Feng li ,WANG Xiao li ,LIU Zheng ,PENG Jian xin. Studies on Multiplication of SfaMNPV by Using Substitutive Host[J]. Journal of Henan Agricultural Sciences, 2003, 0(5): 28-29 |
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| Authors: | HUI Feng li WANG Xiao li LIU Zheng PENG Jian xin |
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| Affiliation: | HUI Feng li 1,WANG Xiao li 1,LIU Zheng 1,PENG Jian xin 2 |
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| Abstract: | The multiplication of SfaMNPV by using its substitutive host Helicoverpa armigera larvae was influenced by factors including different inoculation concentration,larva instar,and temperature.It was favourable to produce SfaMNPV at the temperature of 25 ℃,and inoculation concentration of 1×10 7 PIB/ml,with 3 instar larvae.The output of SfaMNPV from Helicoverpa armigera was averaged 78.34×10 8PIB/g. |
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| Keywords: | Substitutive host Helicoverpa armigera SfaMNPV Multiplication |
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