| 奶牛NUCB2基因突变及其与产奶量关联的分析 |
| |
| 引用本文： | 刘俊霞,李飞,陈宏,雷初朝,任刚.奶牛NUCB2基因突变及其与产奶量关联的分析[J].中国奶牛,2010(4):10-11. |
| |
| 作者姓名： | 刘俊霞 李飞 陈宏 雷初朝 任刚 |
| |
| 作者单位： | 1. 石河子大学动物科技学院,石河子,832003
2. 西北农林科技大学动物科技学院,杨凌,712100 |
| |
| 基金项目： | 国家高技术研究发展计划(863计划)，国家自然科学基金，国家科技支撑计划 |
| |
| 摘 要： | 利用PCR-SSCP和DNA测序技术首次研究了中国荷斯坦牛NUCB2基因的变异。结果表明在NUCB2基因外显子9发现两个沉默突变:27451GA和27472TC,两个碱基变化没有导致氨基酸的改变,但两个位点沉默突变同时发生,表现出3个基因型,即MM、MN和NN,经过对不同基因型与奶牛产奶量性状的关联分析,发现这些突变对奶牛产奶量产生显著影响(P0.05)。
|
| 关 键 词： | NUCB2基因 PCR—SSCP 单核苷酸多态性（SNPs） 产奶量 |
Analysis of Mutation of the NUCB2 Gene and Its Association with Milk Production in Dairy Cow |
| |
| Liu Junxia,Li Fei,Chen Hong,Lei Chuzhao,Ren Gang.Analysis of Mutation of the NUCB2 Gene and Its Association with Milk Production in Dairy Cow[J].China Dairy Cattle,2010(4):10-11. |
| |
| Authors: | Liu Junxia Li Fei Chen Hong Lei Chuzhao Ren Gang |
| |
| Institution: | Liu Junxia1,Li Fei1,Chen Hong2,Lei Chuzhao2,Ren Gang2(1.College of Animal Science , Technology,Shihezi University,Shihezi 832003,2.College of Animal Science , Technology,Northwest A&F University,Yangling 712100) |
| |
| Abstract: | The mutations of NUCB2 gene were studied with PCR-SSCP and DNA sequencing methods in Chinese Holstein.The results showed that two mutant sites with g27451G>A and g27472T>C were detected only at the exon9 of NUCB2 gene.These two silent mutationdid not result in the change of amino acid,but they happened simutaneously.They showed three genotypes,MM,MN and NN.The different genotypes of these two mutation loci were significantly associated with milk production(P<0.05). |
| |
| Keywords: | PCR-SSCP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
