| 甘肃省不同区域当归种子的RAPD分析 |
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| 引用本文： | 周鹤峰,邵敏,姬可平,李啸红,李应东,晋玲.甘肃省不同区域当归种子的RAPD分析[J].安徽农业科学,2010,38(28):15616-15617. |
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| 作者姓名： | 周鹤峰 邵敏 姬可平 李啸红 李应东 晋玲 |
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| 作者单位： | 遵义医学院珠海校区,广东珠海,519041;甘肃省中医学院,甘肃兰州,730000 |
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| 摘 要： | 目的]探讨RAPD技术应用于甘肃省当归Angelicae sinensis（Oliv.）Diels）品种鉴别的可行性。方法]采用改良CTAB法提取甘肃省不同地区的当归种子基因组DNA,利用筛选的引物S61分别对其进行RAPD分析。结果]获得了质量较高的当归种子基因组DNA,引物S61扩增的图谱显示出良好的多态性,且比较稳定,重复性好。结论]该法可为从分子生物学角度鉴别当归提供科学依据。
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| 关 键 词： | 当归种子 DNA提取 改良CTAB法 RAPD |
RAPD Analysis of Six Different Regions Angelicae sinensis Seed in Gansu |
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| Institution: | ZHOU He-feng et al ( Department of bioengineering, Zunyi Medical College Zhuhai Campus,Zhuhai, Guangdong 519041 ) |
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| Abstract: | Objective]To explore the feasibility of using RAPD analysis to identify Angelicae sinensis in Gansu.Methods] The genomic DNAs were extracted from Angelicae sinensis by modified CTAB method and analyzed by RAPD with primer S61.Result]It had obtained high quality team genomic DNA by modified CTAB method.The RAPD map showed that high polymorphism existed in primer S61 was clear and repetitive.Conclusion]The results obtained provided the basis for identifing Angelicae sinensis from the level of molecular biology. |
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| Keywords: | Angelicae sinensis seed DNA extraction modified CTAB RAPD |
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