| 桃PGIP基因及cDNA的克隆及序列分析 |
| |
| 引用本文： | 谌悦,张军科,熊帅. 桃PGIP基因及cDNA的克隆及序列分析[J]. 安徽农业科学, 2009, 37(32): 15738-15740 |
| |
| 作者姓名： | 谌悦 张军科 熊帅 |
| |
| 作者单位： | 西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100;西北农林科技大学园艺学院,农业部西北园艺植物种质资源利用重点开放实验室,陕西杨凌,712100 |
| |
| 摘 要： | 通过PCR扩增了桃的PGIP基因及eDNA序列，并进行了序列之间的比对及分析。结果表明，桃的PGIP基因全长1092bp，而其cDNA序列只有1045bp，PGIP基因中含有一段长147bp的内含子，且内含子符合GT-AG规律。这与其他核果类植物的PGIP基因构成基本相同。其基因序列与GenBank中已登录的3个桃以及其他核果的序列比对，其同源性达92％～99％；与苹果、梨和大桉的同源性分别为85％、84％和8．4％。
|
| 关 键 词： | 桃 PGIP基因 克隆 序列分析 |
Cloning and Sequence Analysis of PGIP gene and cDNA from Prunus persica |
| |
| CHEN Yue et al. Cloning and Sequence Analysis of PGIP gene and cDNA from Prunus persica[J]. Journal of Anhui Agricultural Sciences, 2009, 37(32): 15738-15740 |
| |
| Authors: | CHEN Yue et al |
| |
| Affiliation: | CHEN Yue et al (College of Horticulture,Northwest A & F University,Key Lab of Northwestern Horticultural plant Germplasm Resource Application of Ministry of Agriculture,Yangling,Shaanxi 712100) |
| |
| Abstract: | PGIP gene and cDNA were cloned from Prunus persica and the sequences were compared and analyzed.The result showed that the length of PGIP gene was 1 092 bp,while the length of cDNA was only 1 045 bp.The PGIP gene contained 147 bp length intron,and according to GT-AG law,which basically the same with PGIP gene of other stone fruit plants.its gene sequence and logged 3 peaches in GenBank and other stone fruit were compared the homology can reached to 92%-99%.The homology rates of PGIPs,compared peach with Mal... |
| |
| Keywords: | Prunus persica PGIP Clone Sequence analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
