| 顺丁烯二酸-单酰丙酮酸及反丁烯二酸-单酰丙酮酸的制备 |
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| 引用本文： | 刘冬啟,刘虹,周宁一. 顺丁烯二酸-单酰丙酮酸及反丁烯二酸-单酰丙酮酸的制备[J]. 浙江大学学报(农业与生命科学版), 2005, 31(3): 301-304 |
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| 作者姓名： | 刘冬啟 刘虹 周宁一 |
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| 作者单位： | 1. 中国科学院武汉病毒研究所,湖北,武汉,430071;中国科学院,微生物研究所,北京,100086;中国科学院,研究生院,北京,100039
2. 中国科学院武汉病毒研究所,湖北,武汉,430071 |
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| 基金项目： | 国家自然科学基金，中国科学院"百人计划" |
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| 摘 要： | 来自肺炎克氏杆菌M5a1菌株的龙胆酸1,2-双加氧酶(GDO,EC1.14.13),能够催化龙胆酸产生顺丁烯二酸-单酰丙酮酸(MP).利用龙胆酸1,2-双加氧酶基因工程菌以及MP被酸化时会转化成反丁烯二酸-单酰丙酮酸的特性小量制备了纯度分别为97.7%以及96.4%的顺丁烯二酸-单酰丙酮酸和反丁烯二酸-单酰丙酮酸,为该化合物的制备提供了一条具有一定可行性的生产途径.
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| 关 键 词： | 顺丁烯二酸-单酰丙酮酸 反丁烯二酸-单酰丙酮酸 龙胆酸1 2-双加氧酶 肺炎克氏杆菌M5a1 制备 |
| 文章编号： | 1008-9209(2005)03-0301-04 |
| 修稿时间： | 2004-08-02 |
Production of maleylpyruvate and fumarylpyruvate |
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| LIU Dong-qi,LIU Hong,ZHOU Ning-yi. Production of maleylpyruvate and fumarylpyruvate[J]. Journal of Zhejiang University(Agriculture & Life Sciences), 2005, 31(3): 301-304 |
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| Authors: | LIU Dong-qi LIU Hong ZHOU Ning-yi |
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| Abstract: | Gentisate 1,2-dioxygenase (GDO,EC1.14.13) from Klebsiella pneumoniae M5a1 successfully converted gentisate to maleylpyruvate. This biotransformation was performed by using genetically engineered E.coli strain carrying a gene encoding gentisate 1,2-dioxygenase, and the maleylpyruvate synthesized by this enzyme attained a purity as high as 97.7%. Purity of fumarylpyruvate obtained by acidifying the resulting maleylpyruvate with meta-phosphoric acid was 96.4%. This study thus established a method which is suitable for the production of maleylpyruvate and fumarlpyruvate. |
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| Keywords: | maleylpyruvate fumarylpyruvate entisate 1 2-dioxygenase Klebsiella pneumoniae: M5a1 preparation |
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