以Bar为选择标记的遗传转化体系的建立

Bar基因是转基因商品化作物中应用最多的目标基因，也是水稻遗传转化中应用较多的选择标记基因，因此，建立以Bar基因为选择标记的通用和高效的遗传转化体系非常必要。本研究中，以籼型水稻明恢63为受体，Bar基因作选择标记，采用农杆菌介导的遗传转化法，将Bar基因转入其中，获得了一批转化子，初步建立了稳定、高效的水稻遗传转化体系。随后，以此体系为基础，将装有不同基因的两个载体pBar-1C^＊和pBar-1Ab进行了转化，分别从5400块和4800块愈伤组织中获得156个和115个抗性愈伤，其抗性愈伤率分别为2．8％和2．4％，分化率分别为80．7％和87．8％，由此说明，此转化体系相对稳定，且分化率高、抗性愈伤率也较高，可用于同类选择标记的遗传转化，加快水稻转基因育种步伐。

Development of Transformation System with Selective Marker Bar Gene

Bar gene is widely used in commercialized transgenic crops as a selective marker and also used in rice transformation.Therefore,the establishment of a kind of common and efficiency genetic transformation system is necessary.In the study,by using indica rice Minghui 63 as transformation receptor and Agrobacterium-mediated genetic method,Bar gene was transformed into Minghui 63.Thus,a stable and efficiency transformation system of rice was founded.Subsequently,on the basis of the system,the vectors pBar-1C* and pBar-1Ab were transformed.156 and 115 of resistant calli were obtained from 5 400 and 4 800 calli,respectively.Their transformation efficiency were 2.8% and 2.4%,and differentiation rates were 80.7% and 87.8%,respectively.These results suggest the transformation system is stable as well as efficiency,and good for transformation of rice with Bar gene as selective marker.