| 培养条件对长富2号苹果新梢增殖和生长的影响 |
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| 引用本文： | 何水涛,刘沛镇,崔怀玉,杜建丰,牛小花.培养条件对长富2号苹果新梢增殖和生长的影响[J].果树学报,1996(4). |
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| 作者姓名： | 何水涛 刘沛镇 崔怀玉 杜建丰 牛小花 |
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| 作者单位： | 中国农业科学院郑州果树研究所，西华县林果办 |
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| 摘 要： | 苹果离体新梢在继代培养过程中，以２０００一３０００ｌｘ光照１０ｈ对增殖、生长较好，而以２０００ｌｘ、１０ｈ效果更为理想；昼夜变温２８℃／２４℃与恒温（２６℃）处理增殖和生长无显著差异，但在昼夜２６℃／２２℃、２４℃／２０℃变温下降低了增殖量与有效新梢数；长富２号增殖和生长以ＭＳ＋ＢＡ１ｍｇ·ｌ￣（－１）＋ＩＢＡ０．１ｍｇ·ｌ￣（－１）＋ＣＨ２００ｍｇ·ｌ￣（－１）效果较好，但依继代次数和新梢状态可在ＢＡ０．７一１．０ｍｇ·ｌ￣（－１），ＩＢＡ（或ＩＡＡ）０．１一０．２ｍｇ·ｌ￣（－１）（Ｈ３０一２００ｍｇ·ｌ￣（－１）范围内变动。
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| 关 键 词： | 富士苹果 离体培养 增殖 生长 |
Influence of in vitro Culture Conditions on Fuji Apple Shoot Proliferation and Growth |
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| He Shuitao,Liu Peizhen and Cui Huaiyu Du Jianfen and Niu Xiaohu.Influence of in vitro Culture Conditions on Fuji Apple Shoot Proliferation and Growth[J].Journal of Fruit Science,1996(4). |
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| Authors: | He Shuitao Liu Peizhen and Cui Huaiyu Du Jianfen and Niu Xiaohu |
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| Abstract: | In the course of Fuji apple explant culture in vitro,the growth and proliferation rate was high under2000一3000lx 10h a day. There was no significant difference between treatments of 28℃/24℃ and 26℃/26℃(light/dark).Under 26℃/22℃ and 24℃/20℃,the number of available shoot was reduced.For the shootgrowth and proliferation of Fuji apple the culture medium MS+BA 1 mg·l(-1)+IBA 0.1m·l(-1) +CH200mg·l(-1) was recommanded,which may be varied in a range of BA 0.7一1mg·l(-1),IBA(or IAA)0.1一0.2mg·l(-1),CH30一200 mg·l(-1)depending on the subculture generation and shoot proliferation. |
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| Keywords: | Fuji apple in vitro culture Shoot proliferation Growth |
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