| 如皋鸡EX-FABP基因遗传多态性及其与肌内脂肪含量的相关分析 |
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| 引用本文： | 周琼,常国斌,王克华,窦套存,廖菁,陈国宏. 如皋鸡EX-FABP基因遗传多态性及其与肌内脂肪含量的相关分析[J]. 中国畜牧兽医, 2010, 37(4): 143-145 |
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| 作者姓名： | 周琼 常国斌 王克华 窦套存 廖菁 陈国宏 |
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| 作者单位： | (1.扬州大学动物科学与技术学院， 扬州 225009； 2.中国农业科学院家禽研究所， 扬州 225003） |
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| 摘 要： | 以60周龄和自繁后的12周龄的如皋鸡作为试验材料，采用PCR-SSCP技术对细胞外脂肪酸结合蛋白（EX-FABP）基因进行单核苷酸多态性及遗传特异性分析。结果检测到2个SNP突变，分别为4271 G/A、4281 G/A突变，有3种基因型。最小二乘差异显著性检验结果表明，在60周龄时，AA型含量最高，显著高于AB型（P<0.05）；12周龄时，AB型含量最高，BB型最低；60周龄的肌内脂肪含量显著低于12周龄时的含量（P＜0.05）。
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| 关 键 词： | 细胞外脂肪酸结合蛋白（EX-FABP） 单核苷酸多态性 鸡 肌内脂肪 |
tudy on Single Nucleotide Polymorphism of EX-FABP Gene and its Association with IMF in Ru-gao Chicken |
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| ZHOU Qiong,CHANG Guo-bin,WANG Ke-hua,DOU Tao-cun,LIAO Jing,CHEN Guo-hong. tudy on Single Nucleotide Polymorphism of EX-FABP Gene and its Association with IMF in Ru-gao Chicken[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(4): 143-145 |
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| Authors: | ZHOU Qiong CHANG Guo-bin WANG Ke-hua DOU Tao-cun LIAO Jing CHEN Guo-hong |
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| Affiliation: | (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Poultry lnstitute,Chinese Academy of Agricultural Sciences, Yangzhou 225003, China) |
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| Abstract: | This study was designed to investing the single nucleotide polymorphism and analyzing the genetic specificity of the EX-FABP gene, utilizing the PCR-SSCP technology and DNA sequencing, the 60 weeks of age and reproduction of the 12-week-old Ru-gao chicken as the experimental material. It detected three genotypes, two SNP mutations, 4271 G/A，4281 G/A respectively. The results showed that the content of AA-type was the mostly at the 60-week-old, significantly higher than the AB-type（P<0.05）, using the least-squares significant difference test. At the 12- week-old, the AB-type was in the highest levels, as well as the BB-type was minimum in three genotypes. The intramuscular fat content at 60-week-old was significantly lower than that of the 12-week-old（P＜0.05）. |
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| Keywords: | extracellular fatty acid binding protein (EX-FABP) SNP chicken intramuscular fat |
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