| 一例猪细小病毒病的诊断 |
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| 引用本文： | 林川. 一例猪细小病毒病的诊断[J]. 福建畜牧兽医, 2014, 0(5): 8-9 |
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| 作者姓名： | 林川 |
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| 作者单位： | 福建省泉州市洛江区农业水务局 362011 |
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| 摘 要： | 从取自某猪场表现初产母猪流产胎儿的肾脏、脾脏、肠系膜淋巴结组织,进行研磨后接种猪原代肾细胞,成功分离到一株病毒。该病毒的豚鼠红细胞血凝活性为27,用针对猪细小病毒结构蛋白VP2的特异性引物对分离病毒进行扩增,将扩增结果进行克隆测序,结果经BLAST分析后,证实分离到一株猪细小病毒。
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| 关 键 词： | 猪细小病毒 分离 诊断 |
Diagnosis of porcine parvovirus |
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| Lin Chuan. Diagnosis of porcine parvovirus[J]. Fujian Journal of Animal Husbandry and Veterinary medicine, 2014, 0(5): 8-9 |
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| Authors: | Lin Chuan |
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| Affiliation: | Lin Chuan (Quanzhou luojiang district agriculture water authority, Fujian 362011) |
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| Abstract: | A porcine parvovirus was identified using the porcine primary kidney cells with the mixture of the kidney, spleen, mesen-teric lymph node tissue from sows＆#39; abortion. The hemagglutination titer is 27 using the guinea-pig red blood cell. A target fragments towards the VP2 gene was amplified using PCR method, the sequenced results demonstrated that a porcine parvovirus was identified. |
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| Keywords: | porcine parvovirus isolation diagnosis |
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