兔眼蓝莓‘园蓝’离体培养增殖技术研究

本试验以兔眼蓝莓主栽品种‘园蓝’为试材，研究其离体培养增殖技术。基本培养基为WPM培养基，添加蔗糖20g/L，琼脂10g/L，pH为5.2。实验总体分为两个过程：第一步，在基本培养基中加入ZT (2mg/L, 4mg/L, 6mg/L)或2ip (2mg/L, 5mg/L, 10mg/L)，共6个处理，培养60d后，观察记录蓝莓腋芽萌发情况，WPM ZT 4mg/L更有利于腋芽萌发，各处理中腋芽萌发后生长速度都很慢；第二步，在基本培养基中加入4mg/L的ZT及IAA（0.1mg/L, 0.2mg/L）或IBA（0.1mg/L, 0.2mg/L），共4个处理，培养60d后进行数据统计，发现最佳处理为WPM ZT 4mg/L IBA 0.2 mg/L，并且芽子长势较好。因此，兔眼蓝莓‘园蓝’腋芽诱导和生长的最佳培养基为WPM ZT 4mg/L IBA 0.2 mg/L。

Study on in vitro Multiplication of Rabbit Eye Blueberry Garden Blue

This work studied the in vitro multiplication of rabbit eye blueberry Garden Blue. The basic medium used is WPM plus sucrose 20g/L, agar 10g/L, with pH of 5.2. Two experiments were performed as follows. One was carried out as WPM plus ZT (2mg/L, 4mg/L, 6mg/L) or 2ip(2mg/L, 5mg/L, 10mg/L). Data were collected after 60 days. The results indicate that the best treatment for shoot formation from axillary buds is WPM plus ZT 4mg/L, shoots formed from all treatments grow very slowly. Another was performed as WPM 4mg/L of ZT supplemented with IAA (0.1mg/L, 0.2mg /L) or IBA (0.1mg/L, 0.2mg/L). 60 days later, we found that the best treatment for shoot formation is WPM ZT 4mg/L IBA 0.2 mg/L, and those shoots grow well. As a result, the best medium for shoot formation and growth from axillary buds of Garden Blue is WPM ZT 4mg/L IBA 0.2 mg/L.