| 禽脑脊髓炎琼扩抗原的研制与应用 |
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| 引用本文： | 赵继勋 秦卓明. 禽脑脊髓炎琼扩抗原的研制与应用[J]. 中国家禽, 1997, 0(4): 9-11 |
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| 作者姓名： | 赵继勋 秦卓明 |
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| 作者单位： | 中国农业大学动物医学院 |
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| 摘 要： | 利用禽脑脊髓炎病毒Ｖａｎ Ｒｏｅｋｅｌ株和山东野外分离株，分别脑内接种１日龄ＳＰＦ雏鸡，在隔离器中饲养，以典型发病鸡脑，胃肠和胰腺等制备琼扩抗原。通过特异性检查，效价测定，与进口抗原及血清对比，田间试验等证实，该方法所制备的抗原性能稳定，效价高，特异性强。可广泛用于ＡＥ抗体的普查，适合于评价免疫鸡群抗体的水平。
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| 关 键 词： | 禽脑脊髓炎 琼扩 抗原 SPF 鸡病 |
Preparation and Application of Avian Encephalomyelitis Virus AGP Antigen |
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| Zhao Jixun at el.. Preparation and Application of Avian Encephalomyelitis Virus AGP Antigen[J]. China Poultry, 1997, 0(4): 9-11 |
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| Authors: | Zhao Jixun at el. |
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| Abstract: | ne-day-old SPF chickens were inoculated intracerebrally with avian encephalomyelitis virus Van Roekel strain and shandong-field isolate.Fed in isolator,Chickens with typical symptems were killed and AGP antigen was prepared with brains,Proventriculus,ileums and pancreases.Examined by various methods including specificity determination,Valence determination,Comparison with imported antigen and applications in fields,it was shown that antigen made by this way is stable,sensitive and specific.It can be widely used to estimade AE antibody level |
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| Keywords: | Avian Encephalomyelitis SPF chicken AGP Antigen |
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