| 杨树CDPK基因家族的表达分析及功能预测 |
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| 引用本文： | 张进,李建波,刘伯斌,陈军,卢孟柱.杨树CDPK基因家族的表达分析及功能预测[J].林业科学研究,2014,27(5):604-611. |
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| 作者姓名： | 张进 李建波 刘伯斌 陈军 卢孟柱 |
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| 作者单位： | 林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091 |
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| 基金项目： | “十二·五”863计划课题“杨树分子育种与品种创制”(2011AA100201)；中国博士后科学基金面上资助(2014M550104) |
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| 摘 要： | 为探究CDPK基因在木材形成过程中的作用,本研究在基因组水平上对杨树CDPKs基因家族的成员进行分析,找到32个CDPKs及10个CRKs成员。对CDPK基因家族成员在杨树中表达特性进行了分析,发现其家族成员在不同组织、不同发育阶段以及毛白杨次生维管发育不同时期的表达特性不同。分析杨树CDPK基因家族中3个基因Pt CPK1、Pt CPK6、Pt CPK15,其蛋白质一级结构均存在1个跨膜区,其蛋白定位在细胞膜上。
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| 关 键 词： | 杨树 CDPK 基因家族 基因表达 |
| 收稿时间： | 2013/6/20 0:00:00 |
Expression and Functional Analysis of CDPK Gene Family in Populus |
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| ZHANG Jin,LI Jian-bo,LIU Bo-bin,CHEN Jun and LU Meng-zhu.Expression and Functional Analysis of CDPK Gene Family in Populus[J].Forest Research,2014,27(5):604-611. |
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| Authors: | ZHANG Jin LI Jian-bo LIU Bo-bin CHEN Jun and LU Meng-zhu |
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| Institution: | State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China |
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| Abstract: | Calcium-dependent protein kinases (CDPKs) are important Ca2+ sensors and effectors in Ca2+ signal transduction pathway. They play crucial roles in regulation of plant development and tolerance of various environmental stresses. In this study, a genome-wide analysis of Populus CDPKs was performed, including phylogeny, gene structure and expression patterns. Thirty-two CDPK genes and ten CRK genes were identified. Furthermore, the gene expression profiles showed that a number of Populus CDPK differentially expressed across different tissues and developmental stages. Three Populus CDPK gene, i.e. PtCPK1, PtCPK6 and PtCPK15, were cloned and analyzed, revealing their proteins localized in cell membrane. These results may lay the foundation for functional analysis of Populus CDPK gene family in the future. |
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| Keywords: | Populus CDPK gene family gene expression |
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