我国几种重要松毛虫基因组大小测定

本研究采用流式细胞术,以公鸡(Gallus domesticus)血红细胞DNA含量为标准,测定了我国南方几种重要松毛虫的基因组大小(或称C-值)。结果显示,马尾松毛虫(Dendrolimus punctatus)的基因组大小为563.36±7.26 Mb,其雌、雄2C DNA含量分别为1.175 6±0.016 4和1.128 6±0.013 4 pg;思茅松毛虫(Dendrolimus kikuchii)的基因组大小为719.30±9.70 Mb,其雌、雄2C DNA含量分别为1.449 2±0.021 2和1.442 8±0.018 4 pg;云南松毛虫(Dendrolimus houi)的基因组大小为586.82±8.94 Mb,其雌、雄2C DNA含量分别为1.244±0.018 2和1.156±0.018 4 pg。统计分析结果发现,思茅松毛虫基因组显著大于云南松毛虫和马尾松毛虫基因组,后两者的基因组也存在显著差异,但绝对值相差较小。3种松毛虫雌雄虫的基因组大小之间也存在显著的差异,雌虫基因组大于雄虫。几种松毛虫基因组大小的测定,为进一步比较其分子差异、构建基因组文库和基因组学研究奠定了基础。

Genome Size Determination of Several Dendrolimus Species in China

The genome size of three important Dendrolimus species in South China were measured by flow cytometry, with chicken erythrocyte DNA content as control. The results showed that the genome size of D. punctatus was 563.36±7.26 Mb, and the 2C DNA content of female and male were 1.1756±0.0164 and 1.1286±0.0134 pg, respectively. The genome size of D. kikuchii was 719.30±9.70 Mb, and the 2C DNA content of female and male were 1.4492±0.0212 and 1.4428±0.0184 pg, respectively. The genome size of D. houi was 586.82±8.94 Mb, and the 2C DNA content of female and male were 1.244±0.0182 and 1.156±0.0184 pg, respectively. Statistical analysis indicated that the genome size of D. kikuchii was much greater than that of D. houi and D. punctatus, and the genome size of D. houi was a little larger than that of the D. punctatus. For all the three species, the genome size of the female was larger than that of the male. Genome size determinations of these Dendrolimus species can be used as the basis of further comparison among the molecular differentiation of these closely related and sympatric species, and also provide important information for genomic library construction and sequencing in the future.