| 食品中双歧杆菌检验及鉴定方法研究 |
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| 引用本文： | 胡燕玲,陈亚波,殷欢,梁梅娟.食品中双歧杆菌检验及鉴定方法研究[J].现代农业科技,2021(12). |
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| 作者姓名： | 胡燕玲 陈亚波 殷欢 梁梅娟 |
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| 作者单位： | 中山市食品药品检验所,中山市食品药品检验所,中山市食品药品检验所,中山市食品药品检验所 |
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| 摘 要： | 采用生化鉴定API法、全自动细菌鉴定仪及分子生物学法Riboprinter核糖体分型法对食品中双歧杆菌进行鉴定研究。结果表明:生化鉴定只能得到双歧杆菌属的结果,分子生物学法能准确鉴定到种。采用Riboprinter核糖体分型法能准确鉴定出双歧杆菌的种名,是这次研究中鉴定双歧杆菌的最佳方法。
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| 关 键 词： | 双歧杆菌检验，鉴定，核糖体分型法 |
| 收稿时间： | 2020/12/21 0:00:00 |
| 修稿时间： | 2020/12/21 0:00:00 |
Research on Inspection and Identification Methods of Bifidobacterium in Food |
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| Abstract: | Objective: To explore the identification method of bifidobacteria in food. Methods: Bifidobacteria in food were identified by biochemical identification API, automatic bacterial identification instrument (VITEK2 Copact) and molecular biology method (Riboprinter ribosome typing). Results: The results of three different identification methods showed that the biochemical identification could only obtain the results of bifidobacteria, while the molecular biological method could accurately identify the species. Conclusion: The Riboprinter ribosome typing method can accurately identify the species name of bifidobacteria, which is the best method to identify bifidobacteria in this study. |
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| Keywords: | bifidobacteria test identification ribosome typing |
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